Expression of heat-resistant β-glucosidase in Escherichia coli and its application in the production of gardenia blue  

作　　者：Wenxi Li Jielin Li Ying Xu Yan Huang Shuqi Xu Zirui Ou Xiaoli Long Xinyu Li Xinyu Liu Zening Xiao Jiaqi Huang Weizhao Chen 

机构地区：[1]College of Life Sciences and Oceanography,Shenzhen University,Shenzhen,518060,China [2]College of Materials Science and Engineering,Shenzhen University,Shenzhen,518060,China [3]Health Science Center School of Biomedical Engineering,Shenzhen University,Shenzhen,518060,China [4]College of Mechatronics and Control Engineering,Shenzhen University,Shenzhen,518060,China [5]Shenzhen Key Laboratory for Microbial Gene Engineering,College of Life Sciences and Oceanography,Shenzhen University,Shenzhen,518060,China

出　　处：《Synthetic and Systems Biotechnology》2021年第3期216-223,共8页合成和系统生物技术（英文）

基　　金：funded by Guangdong Provincial Undergraduate Training Program for Innovation and Entrepreneurship(grant No.202110590065);the Undergraduate Academic Competition Project of Shenzhen University(grant No.803-0000290846).

摘　　要：Gardenia blue is a natural blue pigment that is environmentally friendly,non-toxic,and stable.The hydrolysis of geniposide,catalyzed byβ-glucosidase,is a critical step in the production process of gardenia blue.However,β-glucosidase is not resistant to high temperatures,limiting the production of gardenia blue.In this study,we investigated the effectiveness of a heat-resistant glucosidase obtained from Thermotoga maritima in the production of gardenia blue.The enzyme exhibited a maximum activity of 10.60 U/mL at 90℃.Single-factor and orthogonal analyses showed that exogenously expressed heat-resistant glucosidase reacted with 470.3μg/mL geniposide and 13.5μg/mL glycine at 94.2℃,producing a maximum yield of 26.2857μg/mL of gardenia blue after 156.6 min.When applied to the dyeing of denim,gardenia blue produced by this method yielded excellent results;the best color-fastness was achieved when an iron ion mordant was used.This study revealed the feasibility and application potential of microbial production of gardenia blue.

关 键 词：Β-GLUCOSIDASE Gardenia blue GENIPOSIDE Orthogonal experiment 

分 类 号：R378.21[医药卫生—病原生物学]