解脂耶氏酵母作为微生物细胞工厂的应用研究进展  

Advances in the Application of Yarrowia lipolytica as a Microbial Cell Factory

作　　者：赵禹 刘士琦 李建 李圣龙 赵雅坤 肖冬光[1] 于爱群 ZHAO Yu;LIU Shiqi;LI Jian;LI Shenglong;ZHAO Yakun;XIAO Dongguang;YU Aiqun(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;State Key Laboratory of Food Nutrition and Safety,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]省部共建食品营养与安全国家重点实验室,天津300457

出　　处：《食品科学》2021年第19期388-400,共13页Food Science

基　　金：天津市自然科学基金项目(17JCYBJC40800);天津市教委科研计划项目(2017ZD03)。

摘　　要：解脂耶氏酵母(Yarrowia lipolytica)是一种重要的工业微生物菌种,被公认为食品级安全微生物。近年来,随着合成生物学和基因编辑技术的快速发展,科学家们利用合成生物学及基因编辑技术已经成功构建出了能够生产生物化学品、生物燃料、香料、药物、工业酶和药用蛋白等多种高附加值工业产品的解脂耶氏酵母细胞工厂,使得该酵母在食品、药品和生化能源等领域均具有巨大的应用潜力。本文将重点介绍解脂耶氏酵母表达系统、合成生物学元件和基因编辑方法的最新研究进展和应用情况,并对近年来以解脂耶氏酵母作为微生物细胞工厂生产高附加值产品的应用实例进行总结,希望为研究人员进一步利用解脂耶氏酵母进行底盘细胞设计、构建和优化相关合成途径并最终实现目的产物的高效合成提供有用的信息。Yarrowia lipolytica is a widely used industrial microbial strain and is generally recognized as safe(GRAS).In recent years,with the rapid development of biosynthetic techniques and gene editing tools,the novel microbial chassis Y.lipolytica has already been successfully engineered to produce various high value-added industrial products including biochemical products,biofuels,flavorings,pharmaceutical products,industrial enzymes and pharmaceutical proteins,making it a promising candidate for application in the food,pharmaceutical and biochemical energy fields.The purpose of this article is to introduce the readers to the latest progress in the expression system and synthetic biological parts in Y.lipolytica and the application of gene editing tools on it.In addition,we summarize the recent applications of Y.lipolytica as a microbial cell factory for the biosynthesis of some representative industrial products.This article will hopefully provide some useful information for researchers to design,construct and optimize new biosynthetic pathways for high-level production of target products by engineered Y.lipolytica.

关 键 词：解脂耶氏酵母 合成生物学 基因编辑 启动子 终止子 规律间隔成簇短回文重复序列 

分 类 号：Q815[生物学—生物工程]