基于高通量测序分析感染菌核病和健康桑果内生菌群落结构及多样性  

Analysis of Diversity and Community Structure of Endophytes in Sclerotinia sclerotiorum-infected and Healthy Mulberry Fruits by High-Throughput Sequencing

作　　者：彭芳芳 魏召新[1] 李勋兰 罗友进[1] 韩国辉[1] PENG Fangfang;WEI Zhaoxin;LI Xunlan;LUO Youjin;HAN Guohui(Research Institute of Pomology,Chongqing Academy of Agricultural Sciences,Chongqing 401329,China)

机构地区：[1]重庆市农业科学院果树研究所,重庆401329

出　　处：《食品科学》2021年第20期61-68,共8页Food Science

基　　金：重庆市科研机构绩效激励引导专项(cstc2018jxjl0118);重庆市农业科学院青年创新团队项目(NKY-2019QC08);重庆市农发资金-良种创新重大项目(NKY-2016AA002);重庆市特色水果产业技术体系项目(2020(3)03)。

摘　　要：以感染菌核病桑果和健康桑果为材料,采用Illumina MiSeq高通量测序,分析二者内生菌群落结构组成及多样性变化,充分挖掘桑果内生菌资源,探讨伴生菌与菌核病病原菌互作关系,为建立桑果微生物信息系统、桑果菌核病的病原学研究以及生物防治提供参考。结果表明:根据97%的序列相似性水平,可以将细菌和真菌分别归类为281个和259个非重复可操作分类单元(operational taxonomic units,OTU)。内生细菌群落以蓝藻门(Cyanobacteria)、变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)为主,Cyanobacterianorank、Mitochondrianorank 2个细菌属在病果中呈明显下降趋势,敏感性较强。内生真菌群落以子囊菌门(Ascomycota)、Fungiunclassified、担子菌门(Basidiomycota)为主,Fungiunclassified、Basidiomycota受病原菌影响较大,其中Fungiunclassified几乎不存在于感病组,Basidiomycota下降99.07%。内生真菌多样性可明显划分感病果实和健康果实的界限,且感病果实内生真菌多样性远小于健康果实。LEfSe显著差异分析表明,泛菌属(Pantoea)是病果中重要的细菌类群,Ascomycota及其分支锤舌菌纲(Leotiomycetes)、柔膜菌目(Helotiales)、核盘菌科(Sclerotiniaceae)和杯盘菌属(Ciboria),粪壳菌纲(Sordariomycetes),座囊菌纲(Dothideomycetes),炭角菌目(Xylariales)及其分支( Xylariales_f_Xylariales_fam_Incertae_sedis ,Monographella)是健康果实与感病果实间重要显著差异的生物标识。The community composition and diversity of endophytes in Sclerotinia sclerotiorum-infected and healthy mulberry fruits were analyzed using Illumina MiSeq high-throughput sequencing. The results showed that at a 97% sequence similarity level, 281 and 259 bacterial and fungal operational taxonomic units(OTUs) were identified. The endophytic bacterial community mainly included Cyanobacteria, Proteobacteria, Firmicutes and Bacteroidetes, and Cyanobacterianorank and Mitochondrianorank showed a significant downward trend in the infected fruit with high sensitivity;the endogenous fungal community mainly included Ascomycota, Fungiunclassified and Basidiomycota. Fungiunclassified and Basidiomycota were significantly affected by the pathogen. Fungiunclassified was hardly found in the infected group and the abundance of Basidiomycota decreased by 99.07%. The diversity of endophytic fungi could clearly discriminate between the infected and healthy fruits, being much higher in the former than the latter. Linear discriminant analysis effect size(LEfSe) showed that Pantoea was the major bacterial group in the infected fruit. Ascomycota and its branches(Ciboria, Sclerotiniaceae, Helotiales, Leotiomycetes), Sordariomycetes, Dothideomycetes, Xylariales, and its branches(XylarialesfXylarialesIncertaesedis, Monographella) were the significantly differential biomarkers between the healthy and infected fruits.

关 键 词：桑果 内生细菌 内生真菌 群落结构 多样性 

分 类 号：S182[农业科学—农业基础科学]