基于气相色谱的1-MCP检测方法及释放规律研究  

Detection Method and Release Regularity of 1-MCP Based on Gas Chromatography

作　　者：李广胜 邱夕 马骏 李昆仑 陈绍慧 路丹丹[3] LI Guang-sheng;QIU Xi;MA Jun;LI Kun-lun;CHEN Shao-hui;LU Dan-dan(National Engineering and Technology Research Center for Preservation of Agricultural Products(Tianjin),Key Laboratory of Storage of Agicultura Prouduits,Ministry of Agiculture and Rural Affais,Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products,Tianjin 300384,China;Tianjin Agricultural University,Tianjin 300384,China;Tianjin University of Science and Technology,Tianjin 300222,China)

机构地区：[1]国家农产品保鲜工程技术研究中心(天津),农业农村部农产品贮藏保鲜重点实验室,天津市农产品采后生理与贮藏保鲜重点实验室,天津300384 [2]天津农学院,天津300384 [3]天津科技大学,天津300222

出　　处：《保鲜与加工》2021年第10期101-107,共7页Storage and Process

基　　金：天津市农业科学院院长基金(16008)。

摘　　要：以异丁烯作为标准物,绘制标准曲线,采用气相色谱法对1-MCP浓度进行测定。建立条件如下:柱温100℃,载气压力120 kPa,进样器温度130℃,检测器温度150℃,进样量2 mL。此检测条件下,异丁烯和1-MCP浓度与峰面积值均呈良好线性关系,可用于精确检测1-MCP气体的浓度。商品1-MCP释放条件为用水浸湿(或高湿度),释放速率与浸湿程度相关,干燥条件下不释放1-MCP气体。低温(0℃)条件对1-MCP气体的释放产生抑制作用,释放速率显著低于常温(28℃)条件。用保鲜袋包装青皮核桃并进行1-MCP处理(1-MCP药包放置于保鲜袋上部封口处)发现,相同时间内靠近药包放置点的1-MCP积累浓度显著高于远离药包放置点的位置,表现为上部>中部>下部,且10 h内差异均达显著水平。In this paper,the standard curves were drawn using isobutene as the reference material,then the concentrations of 1-MCP were determined by gas chromatography.The experimental conditions were established as follows:column temperature of 100℃,carrier gas pressure of 120 kPa,sample injector temperature of 130℃,detector temperature of 150℃,and the injection volume of 2 mL.Under these conditions,the concentrations and peak areas of isobutene and 1-MCP showed good linear relationships,which could be used for accurate detection of 1-MCP gas concentrations.The 1-MCP could be released when immersed in water(or under high humidity),and the release rates were correlated with the humidity in environment of during the storage of fruits and vegetables,while no 1-MCP gas would be released under dried conditions.The release rates of 1-MCP at low temperature(0℃)were significantly lower than that at normal temperature(28℃).Walnuts were packed with fresh-keeping bags and treated with 1-MCP,and found that the 1-MCP concentrations near the storage point were significantly higher than that far away from the storage point at the same time(top>middle>bottom),and the accumulated concentrations of 1-MCP in different parts of fresh-keeping bag were significantly diverse during 10 hours.

关 键 词：1-MCP 气相色谱(GC) 释放规律 处理浓度 

分 类 号：TS482.295[农业科学—烟草工业]