金针菇MYB转录因子家族成员鉴定及其在子实体形成中的表达模式分析  

Identification of MYB Transcription Factor Family Members of Flammulina filiformis and Analysis of Their Expression Pattern During Fruiting Body Development

作　　者：邓冰 刘宗奇 袁学文 刘靖宇[1,2,3] 孟俊龙 常明昌[1,2,3] 冯翠萍 DENG Bing;LIU Zongqi;YUAN Xuewen;LIU Jingyu;MENG Junlong;CHANG Mingchang;FENG Cuiping(College of Food Science and Engineering,Shanxi Agricultural University,Jinzhong 030801,Shanxi,China;College of Edible Fungi,Shanxi Agricultural University,Jinzhong 030801,Shanxi,China;Collaborative Innovation Center of Quality and Efficiency of Loess Plateau Edible Fungi in Shanxi,Jinzhong 030801,Shanxi,China)

机构地区：[1]山西农业大学食品科学与工程学院,山西晋中030801 [2]山西农业大学食用菌学院,山西晋中030801 [3]山西省食用菌工程技术研究中心,山西晋中030801

出　　处：《食用菌学报》2021年第5期1-11,共11页Acta Edulis Fungi

基　　金：国家自然科学基金(32072647);山西省应用基础研究计划面上青年基金(201901D211380);山西省煤基重点科技攻关项目(FT2014-03-01);山西省重点研发计划重点项目(201603D21106)。

摘　　要：在全基因组中对金针菇(Flammulina filiformis)MYB转录因子进行鉴定,发现其中存在13个MYB转录因子,亚细胞定位和蛋白结构预测发现所有MYB转录因子均为包含螺旋-转角-螺旋结构的核蛋白;对金针菇菌丝、原基、幼嫩子实体和成熟子实体中MYB基因的表达量进行测定,与在菌丝阶段相比,Ff10734、Ff11788和Ff13454基因在原基、幼嫩子实体和成熟子实体阶段显著上调表达;顺式作用元件扫描发现金针菇基因组中存在4353个潜在的MYB靶基因,基因功能分析结果表明MYB靶基因可能涉及细胞器形成、初级代谢产物合成以及次级代谢产物积累等生物学过程。Genome-wide analysis of Flammulina filiformis MYB transcription factors identified 13 MYB genes in F.filiformis.Both subcellular localization prediction and structure prediction showed that all the identified MYB transcription factors were nuclear proteins containing helix-turn-helix structures.The expression levels of Ff10734,Ff11788 and Ff13454 were up-regulated in the primordium,young fruiting body and mature fruiting body stages compared with the mycelium stage.Genome wide cis-acting element scanning revealed 4353 potential MYB target genes.These target genes may participate in organelle formation,primary metabolite synthesis and secondary metabolite accumulation in F.filiformis.

关 键 词：金针菇 MYB转录因子 生长发育 相对表达量 

分 类 号：S646.15[农业科学—蔬菜学]