萎锈灵抗性分子标记及其在食用真菌遗传转化中的应用  

Molecular Markers of Carboxin Resistance and Their Application in Genetic Transformation of Edible Fungi

作　　者：尚俊军 唐利华[1] 万佳宁[1] 杨瑞恒[1] 龚明[1] 汪滢[1] 邹根 鲍大鹏[1] SHANG Junjun;TANG Lihua;WAN Jianing;YANG Ruiheng;GONG Ming;WANG Ying;ZOU Gen;BAO Dapeng(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungi Processing,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China)

机构地区：[1]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海市农业遗传育种重点实验室,上海201403

出　　处：《食用菌学报》2021年第5期132-138,共7页Acta Edulis Fungi

基　　金：上海市自然科学基金(21ZR1456600)。

摘　　要：萎锈灵是一种抑制琥珀酸脱氢酶活性的真菌杀菌剂。琥珀酸脱氢酶复合体中不同亚基的编码基因突变导致其与萎锈灵的亲和性降低,从而使真菌具有萎锈灵抗性表型。笔者综述萎锈灵抗性分子标记的开发历史、作用机制以及在食用真菌遗传转化中的应用,并对其应用前景进行展望。Carboxin is a fungicide that inhibits the activity of succinate dehydrogenase.Mutations in genes coding for fungal succinate dehydrogenase(SDH)subunits leads to a decrease in the affinity of succinate dehydrogenase with carboxin,thereby rendering the mutants to have a carboxin resistance phenotype.The development history,mechanism and application of carboxin resistance markers in the genetic transformation of edible fungi were reviewed,and the application prospect was discussed.

关 键 词：食用真菌 萎锈灵抗性 筛选标记 遗传转化 

分 类 号：S646[农业科学—蔬菜学]