PKCι/YAP1在宫颈癌中的表达及其临床意义  

Expression of PKCι/YAP1 in Cervical Cancer and Its Clinical Significance

作　　者：陈胜民 王国平 林志仁[1] 袁峰[1] CHEN Shengmin;WANG Guoping;LIN Zhiren;YUAN Feng(Department of Radiation Oncology,Affiliated Haikou Hospital of Xiangya Medical College,Central South University,Hainan 570208,China)

机构地区：[1]中南大学湘雅医学院附属海口医院肿瘤放疗科,海口570208

出　　处：《肿瘤防治研究》2021年第10期970-973,共4页Cancer Research on Prevention and Treatment

基　　金：海南省卫生计生行业科研项目(19A200097)。

摘　　要：目的探讨蛋白激酶Cι(PKCι)、Yes相关蛋白1(YAP1)与高危型人乳头瘤病毒(HPV)感染在宫颈癌局部免疫微环境中的相关性。方法选择宫颈正常组织(NCT)、宫颈低级别鳞状上皮内病变(LSIL)、宫颈高级别鳞状上皮内病变(HSIL)以及宫颈鳞状细胞癌(SCC)各80例。收集4组蜡块组织,实时荧光PCR法测定4组组织高危型HPV感染率;免疫组织化学法检测4组组织中PKCι、YAP1、CD4及CD8表达水平并进行相关性以及临床病理分析。结果NCT、LSIL、HSIL以及SCC各组间高危型HPV感染率、PKCι、YAP1、CD4、CD8阳性率差异均有统计学意义(P<0.05);宫颈病变级别与高危型HPV感染、PKCι、YAP1及CD8阳性表达均呈正相关(P<0.05),与CD4阳性表达呈负相关(P<0.05);在SCC中HPV感染、PKCι、YAP1以及CD8阳性表达相互之间的均呈正相关,而与CD4阳性表达均呈负相关(P<0.05);HPV感染、PKCι、YAP1及CD8阳性表达在SCC不同分化程度及有无脉管瘤栓之间差异均有统计学意义(P<0.05)。结论宫颈病变患者常伴有高危型HPV感染与PKCι、YAP1、CD4及CD8表达异常,相互之间可能存在协同作用,造成SCC的局部免疫抑制微环境,为SCC的发病机制研究及其诊治提供可能的策略。Objective To investigate the role of PKCι,YAP1 and high-risk HPV infection in the local immune microenvironment of cervical cancer.Methods We chose 80 cases of normal tissue of the cervix(NCT),cervical low-grade squamous intraepithelial lesion(LSIL),cervical high-grade squamous intraepithelial lesion(HSIL)and early cervical squamous cell carcinoma(SCC)each.Four groups were collected.The infection rate of high-risk HPV in four groups was determined by real-time fluorescence PCR method.The expression levels of PKCι,YAP1,CD4 and CD8 in four groups were measured and correlated by IHC and clinicopathologic features were also analyzed.Results The differences of high-risk HPV infection rate and PKCι,YAP1,CD4,CD8 positive rate among groups of NCT,LSIL,HSIL and SCC had statistical significance(P<0.05).The level of cervical lesions was positively associated with high-risk HPV infection and positive PKCι,YAP1,CD8 expression(P<0.05),while negatively associated with positive CD4 expression(P<0.05).HPV infection and positive PKCι,YAP1,CD8 expression were positively correlated with each other in SCC,while were all negatively correlated with positive CD4 expression(P<0.05).The differences of HPV infection,PKCι,YAP1 and CD8 positive expression were significant in different levels of differentiation and vascular invasion of SCC(P<0.05).Conclusion The patients with cervical lesions are often accompanied by high-risk HPV infection and abnormal expression of PKCι,YAP1,CD4 and CD8,which may have synergistic effects on each other,causing the local immunosuppression microenvironment of SCC.It provides a possible strategy for the study of pathogenesis,diagnosis and treatment of cervical cancer.

关 键 词：宫颈癌 HPV 蛋白激酶Cι Yes相关蛋白1 免疫微环境 

分 类 号：R737.33[医药卫生—肿瘤]