微卫星状态与结直肠癌临床病理特征、外周血T细胞及基因突变的关系  

Relationship between microsatellite status and clinicopathological features,peripheral blood T cells and gene mutations in colorectal cancer

作　　者：钟国方 张航 袁霞[1] ZHONG Guo-fang;ZHANG Hang;YUAN Xia(Department of Oncology,Huizhou Central People’s Hospital,Huizhou 516000,China)

机构地区：[1]惠州市中心人民医院肿瘤内科,广东惠州516000

出　　处：《广东医科大学学报》2021年第5期557-561,共5页Journal of Guangdong Medical University

基　　金：惠州市科技局重大项目(No.2016C0405020)。

摘　　要：目的了解微卫星状态与结直肠癌临床病理特征、外周血T淋巴细胞及基因突变的关系。方法180例结直肠癌患者采用多重荧光PCR毛细管电泳法检测微卫星状态,免疫芯片法检测外周血T淋巴细胞,高通量测序方法检测基因突变状态,分析微卫星状态与结直肠癌临床病理特征、外周血T淋巴细胞及基因突变的关系。结果微卫星高度不稳定(MSI-H)患者<60岁、结肠癌、低分化型、PIK3CA突变、CD3^(+)≥1372个/μL、CD4^(+)≥766个/μL、CD8^(+)≥438个/μL均高于微卫星稳定(MSS)/微卫星低度不稳定(MSI-L)者(P<0.01或0.05)。结论结直肠癌患者微卫星状态与年龄、发病部位、分化程度、外周血T淋巴细胞、PIK3CA基因突变密切相关。Objective To study the relationship between microsatellite status and clinicopathological characteristics,peripheral blood T cells and gene mutations in colorectal cancer(CRC).Methods The microsatellite status,peripheral blood T cells and gene mutations of 180 CRC patients were determined by multiple fluorescent PCR with capillary electrophoresis, immunomicroarray, high-throughput sequencing method, respectively. The relationship between microsatellite status and clinicopathological characteristics, peripheral blood T cells and gene mutations was analyzed. Results Compared with microsatellite stability and microsatellite instability-low, age<60 years, colon cancer, poorly differentiated tumor, PIK3CA mutation, CD3^(+)≥1 372/μL, CD4^(+)≥766/μL, and CD8^(+)≥438/μL were more common in patients with microsatellite instability-high (P<0.01 or 0.05). Conclusion The microsatellite status is closely associated with age, location, differentiation, peripheral blood T lymphocytes, and PIK3CA mutation in CRC patients.

关 键 词：结直肠癌 微卫星不稳定 T淋巴细胞 基因突变 

分 类 号：R935.3[医药卫生—生药学]