Single somatic cell development and differentiation in the gametiphytic blades of Pyropia suborbiculata (Bangiales, Rhodophyta)  

作　　者：Wenxia Guo Hongchang Ding Xinghong Yan 

机构地区：[1]Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai,201306,China [2]Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai,201306,China

出　　处：《Aquaculture and Fisheries》2021年第5期479-484,共6页渔业学报（英文）

基　　金：The study was supported in part by the National Key Research and Development Program of China(2018YFD0900606);National Natural Science Foundation of China(31072208);Major Science and Technology Specific Program of Zhejiang Province(2016C02055-6);Science and Technology Planning Project of Jiangsu Province,China(BE2018335);Open Program of Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province(2017fjscq02).

摘　　要：Conchospores released from a wild-type strain of Pyropia suborbiculata were cultured in the laboratory for 20-80 days.Mother blades of different ages were enzymatically isolated to obtain single cells,which then developed into different types of regenerative plants within a liquid medium.These regenerative plant types includes:normal blades,abnormal blades,cell-masses,and sexual cell-masses.The age of mother blades distinctly affected the number and proportion of each type of regenerative plants.When the age of mother blades increased from 20 to 80 days,the proportion of normal blades in the isolated cells sharply decreased,the percentage of abnormal blades increased,and the proportion of cell-masses was relatively unchanged.After 40 days of cultivation,sexual cell-masses appeared in regenerated plants derived from the single blade cells of the PS-WT strain,and gradually increased with mother cell age.In addition,the proportion of normal blades decreased in regenerated plants sourced from the basal to apical parts of the same alga,while the proportion of abnormal blades and cell-masses increased.In summary,the isolated cells of the gametophytic blade of P.suborbiculata developed into different types of regenerated plantlets in vivo due to the different stages of cell differentiation.We preliminarily concluded that the differentiation from conchospores to the sexual cells could be divided into at least seven different groups.

关 键 词：Pyropia suborbiculata Development DIFFERENTIATION Single somatic cells Regenerative plants 

分 类 号：S917.3[农业科学—水产科学]