黄药叶总黄酮提取工艺优化及其 抗氧化活性研究  

Optimization of Extraction and Antioxidant Activity of Total Flavonoids from Premna cavaleriei Levl.Leaves

作　　者：桂利利 吴正坤 余惠凡 李飞 GUI Li-li;WU Zheng-kun;YU Hui-fan;LI Fei(Hubei Key Laboratory of Wudang Local Chinese Medicine Research,Department of Pharmacology,Hubei University of Medicine,Shiyan 442000,Hubei,China;School of Pharmaceutical Sciences,Hubei University of Medicine,Shiyan 442000,Hubei,China)

机构地区：[1]湖北医药学院药学院武当特色中药研究湖北省重点实验室,湖北十堰442000 [2]湖北医药学院生物医药研究院,湖北十堰442000

出　　处：《食品研究与开发》2021年第19期100-108,共9页Food Research and Development

基　　金：湖北省教育厅重点项目(D20192101);湖北省卫生健康委青年人才项目(WJ2021Q010);十堰市市级引导性科研项目(21Y10);湖北医药学院人才启动金项目(2018QDJZR33)。

摘　　要：采用超声波辅助提取法提取黄药叶总黄酮,通过单因素试验和正交试验优化黄药叶总黄酮提取工艺,并探讨其体外抗氧化活性。结果表明,黄药叶总黄酮最佳提取工艺参数为乙醇体积分数70%,提取次数3次,提取时间40 min,料液比1∶30(g/mL),此条件下黄药叶总黄酮得率为(6.18±0.13)%。体外抗氧化试验结果表明,黄药叶总黄酮提取物对羟自由基、DPPH自由基和超氧阴离子自由基均具有一定的清除能力,相应的半数清除浓度(inhibitory concentration,IC_(50))为484.12、18.27、46.88μg/mL,分别为L-抗坏血酸的1.50、4.50、2.51倍,黄药叶总黄酮提取物铁离子还原能力略低于L-抗坏血酸。Single factor test and orthogonal test were used to optimize ultrasound-assisted extraction of total flavonoids from Premna cavaleriei Levl.leaves.The antioxidant activity in vitro of the leaves was analyzed.Optimized extraction parameters were established as follows:ethanol concentration 70%;extraction times 3;extraction time 40 min;solid/liquid ratio 1∶30(g/mL).Under these conditions,total flavonoid yield was(6.18±0.13)%.The results of antioxidant experiments showed that the total flavonoids extracted from Premna cavaleriei Levl.leaves had certain scavenging rate of hydroxyl radicals,DPPH radicals and superoxide anion radicals.The corresponding inhibitory concentration(IC_(50))was 484.12,18.27 and 46.88μg/mL,respectively and were 1.50,4.50 and 2.51 times that of L-ascorbic acid,respectively.The ferric ion reducing ability of total flavonoids from Premna cavaleriei Levl.leaves was slightly lower than that of L-ascorbic acid.

关 键 词：黄药叶 总黄酮 提取工艺 正交试验 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]