富集核糖体蛋白检测大肠杆菌  

Detection of Escherichia coli by the Enrichment of Ribosomal Protein

作　　者：刘鹏[1] 魏纪平[1] 李超 袁文蛟[1] 刘皓[1] 李达[1] LIU Peng;WEI Ji-ping;LI Chao;YUAN Wen-jiao;LIU Hao;LI Da(Tianjin Modern Vocational Technology College,Tianjin 300350,China)

机构地区：[1]天津现代职业技术学院,天津300350

出　　处：《食品研究与开发》2021年第19期187-191,共5页Food Research and Development

基　　金：天津市科技计划项目(17YFFCZC00220、18YFZCNC01140);天津市津南区科技计划项目(20190112)。

摘　　要：致病菌的快速检测已成为研究热点,该文通过合成Fe_(3)O_(4)-COOH@MIL-101纳米粒子,利用该材料的带电性和介孔的富集能力,提高基质辅助激光解吸飞行时间质谱对核糖体蛋白的灵敏性和检测限,并利用核糖体蛋白的保守性完成对大肠杆菌O157∶H7的鉴别。The rapid detection of pathogenic bacteria has become a topic spot.In the study,Fe_(3)O_(4)-COOH@MIL-101 nanoparticles were synthesized to improve the sensitivity and detection limit of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS/MS)to ribosomal proteins using its charged property and mesoporous enrichment ability.The conservation of ribosomal proteins had been used to identify E.coli O157∶H7.

关 键 词：富集 核糖体 蛋白 检测 大肠杆菌O157∶H7 致病菌 

分 类 号：TS207.4[轻工技术与工程—食品科学]