茄子SmCOL5基因在花青素生物合成中的功能分析  

Functional characterization of SmCOL5 gene in anthocyanin biosynthesis in Solanum melongena.L

作　　者：胡艺伟 李大露 席浩淳 何永军 陈火英[1] 刘杨[1] HU Yiwei;LI Dalu;XI Haochun;HE Yongjun;CHEN Huoying;LIU Yang(College of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《上海农业学报》2021年第5期1-5,共5页Acta Agriculturae Shanghai

基　　金：上海市农委科技兴农项目(2019-02-08-00-08-F01106);国家自然科学基金项目(31872944)。

摘　　要：通过同源克隆的方法从茄子中分离出SmCOL5基因,并对其蛋白功能进行分析。结果表明:SmCOL5蛋白定位于细胞核中;SmCOL5蛋白可与组成型光形态建成SmCOP1蛋白互作,说明SmCOL5蛋白的功能受到光的调控;且SmCOL5蛋白能与花青素生物合成途径中重要的结构基因SmF3H、SmCHS的启动子结合。推测SmCOL5基因在接收到光受体信号后参与茄子花青素生物合成的调控途径。SmCOL5 gene was isolated from eggplant by homologous cloning,and its protein function was analyzed.The results showed that SmCOL5 protein was localized in the nucleus;Smcol5 protein could interact with constitutive photomorphogenesis SmCOP1 protein,indicating that the function of SmCOL5 protein was regulated by light;SmCOL5 protein could bind to the promoters of important structure genes SmF3H and SmCHS in anthocyanin biosynthesis pathway.It was speculated that SmCOL5 gene was involved in the regulation of anthocyanin biosynthesis in eggplant after receiving photoreceptor signal.

关 键 词：茄子 花青素 SmCOL5基因 酵母双杂交 酵母单杂交 

分 类 号：S641.1.03[农业科学—蔬菜学]