豫玉22玉米杂交种子纯度的SSR和SNP分子比较鉴定  

Comparative Identification for Hybrid Seed Purity of Yuyu 22 Maize by SSR and SNP

作　　者：尹祥佳[1] 李晶 王雅琳[1] 王剑虹[1] 翟琛 郝楠[1] 焦珍珍 YIN Xiangjia;LI Jing;WANG Yalin;WANG Jianhong;ZHAI Chen;HAO Nan;JIAO Zhenzhen(Lanzhou Vocational and Technical College,Lanzhou Gansu 730070;Beijing Tongzhou International Seed Science and Technology Co.,Ltd.,Beijing 101100)

机构地区：[1]兰州职业技术学院,甘肃兰州730070 [2]北京通州国际种业科技有限公司,北京101100

出　　处：《现代农业科技》2021年第20期12-15,共4页Modern Agricultural Science and Technology

基　　金：2019年度兰州市科技发展指导性计划项目(2019-ZD-156);兰州职业技术学院2020—2021年度院内科研项目(2020XY-9)。

摘　　要：采用SSR荧光标记和SNP分子标记的KASP技术对豫玉22玉米杂交种子进行基因组DNA提取、引物筛选、基因型分析、纯度鉴定,以期为快速有效鉴定玉米种子纯度提供依据。结果表明:豫玉22的SSR特异性引物为umc2007y4和bnlg161k8,纯度鉴定结果为97.92%;SNP特异性引物为MaSNP64和MaSNP245,纯度鉴定结果为98.44%。因此,SSR和SNP分子标记是鉴定豫玉22玉米杂交种子纯度的最佳方法。Using KASP technology by SSR fluorescent labeling and SNP molecular marker,the genomic DNA of Yuyu 22 maize hybrid seeds was extracted,and the primers were screened,genotypes were analyzed and purity was identified,in order to provide basis for rapid and effective identification of maize seed purity.The results showed that the SSR specific primers of Yuyu 22 were umc2007y4 and bnlg161k8,and the purity was 97.92%.The SNP specific primers were MaSNP64 and MaSNP245,and the purity was 98.44%.Therefore,SSR and SNP molecular markers were the best methods to identify the purity of Yuyu 22 maize hybrid seeds.

关 键 词：玉米 豫玉22 纯度鉴定 SSR SNP 

分 类 号：S513[农业科学—作物学]