KDM5A对卵巢癌SKOV3/PTX细胞增殖的影响  

Effect of KDM5A on proliferation of ovarian cancer SKOV3/PTX cells

作　　者：冯同富[1] 汪黎明[1] 徐真[1] 张伶俐[1] FENG Tong-Fu;WANG Li-Ming;XU Zhen(Department of Gynecology,Hubei Provincial Maternal and Child Health Care Hospital,Wuhan,Hubei 430070,China)

机构地区：[1]湖北省妇幼保健院妇科,湖北武汉430070

出　　处：《中国妇幼保健》2021年第20期4804-4807,共4页Maternal and Child Health Care of China

基　　金：湖北省自然科学基金知识创新专项(2018CFC840);湖北省卫生和计划生育委员会联合基金立项项目(WJ2018H0158);武汉市中青年医学骨干人才培养工程([2018]116)。

摘　　要：目的分析KDM5A对卵巢癌SKOV3/PTX细胞增殖的影响。方法构建KDM5A基因表达沉默的SKOV3/PTX细胞系,分为沉默组(SKOV3/PTX-KDM5Ai)、阴性对照组(SKOV3/PTX-NC)及空白对照组(SKOV3/PTX),应用Western blot法验证KDM5A基因的沉默效果,CCK-8法检测3组细胞的增殖活性,软琼脂克隆实验检测3组细胞的克隆能力,流式细胞术检测3组细胞的周期分布比例,UALCAN数据库分析KDM5A与增殖分子CHD4、GSK3B表达的相关性。结果沉默组SKOV3/PTX细胞系中KDM5A蛋白表达水平为(0.20±0.01),明显低于阴性对照组(0.74±0.02)和空白对照组(0.75±0.03),差异均有统计学意义(t=46.667,P<0.01;t=47.607,P<0.01)。培养24 h,沉默组细胞的增殖活性明显低于阴性对照组和空白对照组(t=3.820,P<0.01;t=4.511,P<0.01)。沉默组细胞的克隆形成数为(265.43±13.14)个,阴性对照组细胞的克隆形成数为(384.37±19.23)个,空白对照组细胞的克隆形成数为(368.26±11.41)个。沉默组细胞的克隆形成数明显低于阴性对照组和空白对照组,差异均有统计学意义(t=9.894,P<0.01;t=8.564,P<0.01)。沉默组细胞的G0/G1期比例明显增高,与阴性对照组和空白对照组比较,差异均有统计学意义(t=8.922,P<0.01;t=10.529,P<0.01);阴性对照组和空白对照组细胞的G0/G1期比例比较差异无统计学意义(t=1.607,P>0.05)。KDM5A与CHD4、GSK3B均存在明显的相关性(r=0.742,r=0.603)。结论 KDM5A和卵巢癌SKOV3/PTX细胞增殖密切相关,干扰SKOV3/PTX细胞中KDM5A的表达可明显抑制细胞的增殖活性和克隆能力,但其参与和调控这一过程的具体机制还有待进一步研究。Objective To analyze the effect of KDM5 A on proliferation of ovarian cancer SKOV3/PTX cells. Methods A SKOV3/DDP cell line with silencing KDM5 A gene expression was successfully established, then the cells were divided into silence group(SKOV3/PTX-KDM5 Ai),negative control group(SKOV3/PTX-NC),and blank control group(SKOV3/PTX),Western blot was used to verify the silencing effect of KDM5 A gene, CCK-8 assay was used to detect cell proliferative activity, soft agar cloning test was used to detect clone ability, flow cytometry was used to detect the distribution proportions of cell cycles, UALCAN software was used to analyze the correlations between KDM5 A and expressions of CHD4 and GSK3 B. Results The expression rate of KDM5 A protein in SKOV3/PTX cell line in silence group was(0.20±0.01),which was statistically significantly lower than those in negative control group(0.74±0.02) and blank control group(0.75±0.03),there were statistically significant differences(t=46.667,P<0.01;t=47.607,P<0.01). After culture for 24 hours, cell proliferative activity in silence group was statistically significantly lower than those in negative control group and blank control group(t=3.820,P<0.01;t=4.511,P<0.01). The clone formation numbers in silence group, negative control group, and blank control group were(265.43±13.14),(384.37±19.23),and(368.26±11.41),respectively. The clone formation number in silence group was statistically significantly lower than those in negative control group and blank control group(t=9.894,P<0.01;t=8.564,P<0.01). The proportion of cells of G0/G1 phase increased significantly, compared with negative control group and blank control group, there were statistically significant differences(t=8.922,P<0.01;t=10.529,P<0.01),there was no statistically significant difference in the proportion of cells of G0/G1 phase between negative control group and blank control group(t=1.607,P>0.05). KDM5 A was significantly correlated with CHD4 and GSK3 B(r=0.742,r=0.603). Conclusion KDM5 A is closely correlated with pr

关 键 词：卵巢肿瘤 KDM5A 增殖 

分 类 号：R737.31[医药卫生—肿瘤]