Inhibition of Botrytis cinerea and control of gray mold on table grapes by calcium propionate  

作　　者：Chenxu Sun Chuanxi Zhu Ying Tang Dandan Ren Yunfei Cai Ganghan Zhou Yiwen Wang Ling Xu Pinkuan Zhu 孙晨旭;朱传玺;唐瑛;任丹丹;蔡云飞;周港涵;王奕文;许玲;朱品宽(School of Life Sciences,East China Normal University,Shanghai,China)

机构地区：[1]School of Life Sciences,East China Normal University,Shanghai,China

出　　处：《Food Quality and Safety》2021年第3期279-290,共12页食品品质与安全研究（英文版）

基　　金：supported by the grants from Shanghai Municipal Science and Technology Commission,China(No.18391901400);the National Key Research and Development Program of China(2016YFD0400105);the National Natural Science Foundation of China(No.32061133006);the Shanghai Municipal Agricultural and Rural Committee(2019-02-08-00-02-F0U46),China。

摘　　要：Objectives:The gray mold fungus Botrytis cinerea(B.cinerea)infects a wide range of crops before and after harvest,causing huge losses worldwide.Inhibition mechanisms of B.cinerea in vitro and in plants by calcium propionate(CP),generally recognized as a safe substanee,are described in this study.Materials and methods:Wild-type and transgenic mutant strains of B.cinerea were used in the study to evaluate the effects of CP on fun gal growth and developme nt in vitro.Plant materials in cludi ng tomato leaves and table grapes were tested for con trolling efficie ncy of CP agai nst gray mold deterioration in vivo.Results:Mycelial growth of B.cinerea was inhibited by CP in a dose-dependent manner with occasional disruption of hyphal tips,causing cellular collapse and efflux of cell contents.Staining with fluorescein diacetate and propidium iodide indicated that CP decreased fungal cell viability.Inhibition efficiency of CP against B.cinerea was enhaneed by reducing pH.In contrast,the veil mutant,which exhibited deficiency in acid production,was more resista nt to CP,suggesti ng that inhibition of B.cinerea by CP is enhanced by the acidification ability of the fungus itself.Additionally,CP inhibited infection cushion development by germlings of B.cinerea.Infection assays with tomato leaves and table grapes showed that CP inhibited decay development in both host tissues.Moreover,application of CP on grapes 3 days prior to harvest could contribute to management of deterioration caused by spontaneous fungal diseases during storage.Conclusion:CP can suppress hyphal growth,inhibit infection cushion development,and reduce the virulence of B.cinerea.CP is thus promising for practical management of gray mold in fruit crops and merits further evaluation.

关 键 词：Necrotrophic pathogen virulence factor infection cushion green fluorescent protein generally recognized as safe. 

分 类 号：S43[农业科学—农业昆虫与害虫防治]