Analysis of the Microbiome Based on 16S rRNA Gene Signature in Women with Preterm versus Term Birth  

作　　者：Jiao Yu Ting Peng Jiong Lu Xiao-Tian Li Rong Hu 

机构地区：[1]Obstetrics and Gynecology Hospital of Fudan University,Shanghai 200090,China [2]Shiji Medical Laboratory,Shanghai 200093,China

出　　处：《Reproductive and Developmental Medicine》2021年第2期81-89,共9页生殖与发育医学（英文版）

基　　金：This study was supported by the Program of Shanghai Leading Talent(2012),Shanghai Key Program of Clinical Science and Technology Innovation(17411950500,17411950501,18511105602);National Science Foundation of China(81741047,81971411,81571460);Shanghai Medical Center of Key Programs for Female Reproductive Diseases(2017ZZ01016);National Key Basic Research Plan of China(973 Plan)(2015CB943300);National Key R&D Program of China(2016YFC1000403);The Major Program of the National 13th Five-Year Plan of China(2016YFC1000400).

摘　　要：Objective: To characterize and compare the microbiome signature in the maternal, intrauterine, and fetal environments and the associated bacterial species in women who experienced preterm birth and term birth.Methods: A total of 140 women with singleton pregnancies were enrolled in this study. Among them, 31 experienced spontaneous preterm delivery (gestational age < 37 weeks), and 28 of them experienced vaginal delivery at term. Maternal peripheral blood, saliva, and vaginal discharge samples and fetal membrane, amniotic fluid, and cord blood samples were collected immediately after delivery under sterile conditions. DNA was isolated from the fetal membrane and umbilical cord blood samples, and the V3-V4 region of the bacterial 16S rRNA gene was sequenced. The sequence data were quality-filtered, chimera-checked, and organized into operational taxonomic units (OTUs) based on phylogeny. Principal coordinate analysis of beta diversity measures was used for visualization. The linear discriminant analysis effect size (LEfSe) algorithm and Wilcoxon test were used to differentiate the microbiomes found in the fetal membranes and cord blood in the cases of preterm birth.Results: OTU analysis based on the 16S rRNA gene showed similar microbiomes in the maternal peripheral blood, amniotic fluid, fetal membranes, and cord blood. However, the LEfSe algorithm revealed significantly different bacterial compositions in the fetal environment between the preterm and term groups, with some of the bacterial species originating from the maternal peripheral blood or saliva.Conclusions: The bacteria in the intrauterine and fetal environments may originate from other body sites through hematogenous transmission, and may cause the occurrence of preterm birth.

关 键 词：Hematogenous Transmission MICROBIOME Preterm Birth 16S rRNA Gene 

分 类 号：R714.21[医药卫生—妇产科学]