微卫星分子标记技术在大黄鱼增殖放流效果评估中的应用  

Application of microsatellite markers for evaluating the effect of restocking enhancement in Larimichthys crocea

作　　者：吴利娜 张凝鋆 孙松 袁吉贵 陈佳 李苗苗 林楠 游宇 王伟继[3] 丁少雄 WU Lina;ZHANG Ningjun;SUN Song;YUAN Jigui;CHEN Jia;LI Miaomiao;LIN Nan;YOU Yu;WANG Weiji;DING Shaoxiong(State Key Laboratory of Large Yellow Croaker Breeding,Ningde 352103,China;College of Ocean and Earth Sciences,Xiamen University,Xiamen 361002,China;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;Fujian Provincial Fisheries Technology Extension Center,Fuzhou 350002,China)

机构地区：[1]大黄鱼育种国家重点实验室,福建宁德352103 [2]厦门大学海洋与地球学院,福建厦门361002 [3]中国水产科学研究院黄海水产研究所,山东青岛266071 [4]福建省水产技术推广总站,福建福州350002

出　　处：《中国水产科学》2021年第9期1100-1108,共9页Journal of Fishery Sciences of China

基　　金：国家重点研发计划项目(2018YFC1406301);大黄鱼育种国家重点实验室开放课题基金项目(LYC2017RS04).

摘　　要：为研究微卫星分子标记在大黄鱼(Larimichthys crocea)增殖放流效果评估中的适用性,本研究利用8个多态性丰富的微卫星位点,对100尾放流大黄鱼亲鱼以及官井洋海域回捕的295尾大黄鱼样本进行亲子鉴定,并提出了一种基于微卫星分子标记的大黄鱼回捕效果评估方法。8个微卫星位点共检测到78个等位基因,其观测杂合度为0.511~0.939(平均值0.764),期望杂合度为0.643~0.897(平均值0.781),多态性信息含量为0.587~0.883(平均值为0.751);通过Cervus 3.0.7软件模拟分析得到,在置信度大于95%的情况下,8个微卫星位点的个体累积排除率达到99.99%以上水平;2019年7月从官井洋海域回捕到的190尾大黄鱼中,检测出5尾与放流亲本存在亲子关系的个体,占回捕样本总数的2.63%。推算本次富发放流大黄鱼在回捕样本中所占比例为60.53%左右,进一步推算出7月富发公司放流大黄鱼的回捕率为0.0038%左右。8月、9月的105尾回捕样品中未能检出标记个体。本研究构建的大黄鱼亲子鉴定技术体系不仅可为三沙湾大黄鱼增殖放流效果评估工作提供可靠的技术支撑,亦可为三沙湾大黄鱼保护区的科学管理提供参考,此外本研究对于我国其他海水鱼类的增殖放流评估工作的开展也将有重要的参考价值。Large yellow croaker(Larimichthys crocea)is an important commercial marine fish endemic to China.It has long been ranked as one of four major seafood items in China.Unfortunately,overfishing,pollution of the ocean environment,and other factors have led to a severe decline in the natural resources of large yellow croaker.Enhancement release of large yellow croaker has been conducted since 1987 and is considered an effective fishery management measure.Although the traditional tagged method can indirectly assess the effect of enhancement release,it has numerous disadvantages,such as high cost,complicated operation,tags easily falling off,and fish body damage,which obstruct the effectiveness of the assessment.Furthermore,few strategies and methods have previously been proposed to precisely assess the effect of enhancement release of large yellow croaker.Through mixing“labeled”individuals with a known parental origin and the released population at a certain proportion,and identifying“labeled”individuals through parentage identification after recapturing,microsatellite marker technology calculates the rate of recapture with a higher precision,and is becoming more popular in the enhancement release assessment.To assess the applicability of microsatellite markers in evaluating the effect of enhancement releasing programs in Larimichthys crocea,this study used a total of 8 microsatellite loci with rich polymorphism to conduct parentage identification for 100 parents of released offspring and 295 individuals recaptured from Guanjingyang sea water.In addition,this study also proposed a method for evaluating the recapture effect of large yellow croaker enhancement programs based on microsatellite molecular markers.In total,78 alleles were detected at the 8 microsatellite loci.The values of observed heterozygosity and excepted heterozygosity were 0.511–0.939(mean 0.764)and 0.643–0.897(mean 0.781),respectively,and the polymorphism information content of the 8 loci was 0.587–0.883(mean value was 0.751).The results

关 键 词：大黄鱼 微卫星分子标记 增殖放流 亲子溯源 

分 类 号：S931[农业科学—渔业资源]