珠芽黄魔芋组织培养与快繁技术研究  

Study on Tissue Culture and Rapid Propagation Technology of Amorphophallus bulbifer

作　　者：杨宝明[1] 苏艳[2] 李永平[1] 杨超振 黄玉玲[1] 李迅东[1] 尹可锁[1] 王丽花[2] 张艺萍[2] Yang Baoming;Su Yan;Li Yongping;Yang Chaozhen;Huang Yuling;Li Xundong;Yin Kesuo;Wang Lihua;Zhang Yiping(Agricultural Environment and Resources Institute,Yunnan Academy of Agricultural Sciences,Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province,Kunming,Yunnan 650205,China;Institute of Flower,Yunnan Academy of Agricultural Sciences,Supervision and Testing Centre for Flowers of the Ministry of Agriculture and Rural Affairs,National Engineering Research Center For Ornamental Horticulture,Kunming,Yunnan 650205,China;Research Institute for Tropical Eco-agricultural Sciences,Yunnan Academy of Agricultural Sciences,Yuanmou,Yunnan 651300,China)

机构地区：[1]云南省农业科学院农业环境资源研究所云南省农业跨境有害生物绿色防控重点实验室,云南昆明650205 [2]云南省农业科学院花卉研究所农业农村部花卉产品质量监督检验测试中心(昆明)国家观赏园艺工程技术研究中心,云南昆明650205 [3]云南省农业科学院热区生态农业研究所,云南元谋651300

出　　处：《广西林业科学》2021年第5期534-538,共5页Guangxi Forestry Science

基　　金：科技部、财政部国家科技资源共享服务平台项目(NCGRC-2020-47)。

摘　　要：为解决珠芽黄魔芋(Amorphophallus bulbifer)产业高质量规模化发展的种苗瓶颈问题,以MS为基本培养基、珠芽黄魔芋块茎为外植体,开展外植体灭菌、愈伤组织诱导、不定芽增殖和生根壮苗试验,筛选珠芽黄魔芋的最佳组培快繁方法。结果表明,最佳外植体灭菌方法为0.3%害剋溶液灭菌40 min,0.1%HgCl_(2)溶液灭菌5 min;最佳愈伤组织诱导培养基为MS+6-BA 1.5 mg/L+NAA 0.5 mg/L;最佳不定芽增殖培养基为MS+6-BA 2.5 mg/L+NAA 0.2 mg/L;最佳生根培养基为MS+NAA 0.5 mg/L。构建的珠芽黄魔芋无性快繁技术体系在生产中效果明显。In order to solve bottleneck problem of high-quality and large-scale development of Amorphophallus bulbifer industry,taking MS as basic medium and using tubers of A.bulbifer as explants,experiments of explant sterilization,callus induction,adventitious bud proliferation and rooting were carried out,and the best tissue culture and rapid propagation method was selected.Results showed that the best method of explant sterilization was 0.3%hydrocare solution for 40 min and 0.1%HgCl_(2)solution for 5 min.The best medium for callus induc⁃tion was MS+6-BA 1.5 mg/L+NAA 0.5 mg/L.The best medium for adventitious bud proliferation was MS+6-BA 2.5 mg/L+NAA 0.2 mg/L.The best rooting medium was MS+NAA 0.5 mg/L.Asexual rapid prop⁃agation technology system of A.bulbifer was established,and production reaction effect was obvious.

关 键 词：组织培养 快繁技术 珠芽黄魔芋 

分 类 号：X123[环境科学与工程—环境科学]