lncRNA PEBP1P2通过CARD10基因调控NF-κB信号通路对肾细胞癌细胞增殖和迁移的影响  

Effect of lncRNA PEBP1P2 on the proliferation and migration of renal cell carcinoma cells by regulating CARD10 gene and regulating NF-κB signaling pathway expression

作　　者：叶志华 付金伦 桂定文[1] 罗帅 王静[2] 王小英[1] Ye Zhihua;Fu Jinlun;Gui Dingwen;Luo Shuai;Wang Jing;Wang Xiaoying(Department of Urology,Huangshi Central Hospital of Edong Healthcare Group(Affiliated Hospital of Hubei Polytechnic University),Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention,Huangshi 435000,China;Department of Surgery,Huangshi Second Hospital of Edong Healthcare Group,Huangshi 435000,China)

机构地区：[1]鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)泌尿外科,肾脏疾病发生与干预湖北省重点实验室,435000 [2]鄂东医疗集团黄石市第二医院外科,435000

出　　处：《国际外科学杂志》2021年第9期595-599,I0004,共6页International Journal of Surgery

基　　金：湖北省卫生健康科研基金(WJ2021Q006)。

摘　　要：目的:观察长链非编码RNA(lncRNA)PEBP1P2在肾细胞癌组织中的表达及对肾细胞癌细胞增殖和迁移的影响。方法:采用实时定量聚合酶链反应(qPCR)检测51例肾细胞癌组织及肾细胞癌细胞株中PEBP1P2的表达。以PEBP1P2表达最低的A498细胞为转染对象,设转染PEBP1P2质粒的细胞为PEBP1P2组,转染阴性对照质粒的细胞为NC组。qPCR检测两组细胞PEBP1P2的表达。MTT法和Transwell迁移实验检测肾细胞癌细胞的增殖能力和迁移能力。qPCR和Western blotting法分别检测半胱氨酸天冬氨酸蛋白酶招募域家族分子10( CARD10)基因及NF-κB通路蛋白的表达。计量资料以均数±标准差(Mean±SD)表示,组间比较采用 LSD- t检验。 结果:肾细胞癌组织中PEBP1P2的表达低于癌旁组织(t=4.89, P<0.01)。肾细胞癌细胞中PEBP1P2的表达低于正常肾小管上皮细胞(P<0.01)。PEBP1P2组和NC组A498细胞中PEBP1P2表达分别为(11.01±1.26)和(1.06±0.19),PEBP1P2组明显高于NC组(t=7.81, P<0.01)。过表达PEBP1P2可显著抑制肾细胞癌细胞的增殖能力(P<0.05)和迁移能力(t=3.65, P<0.05)。过表达PEBP1P2可显著抑制肾细胞癌A498细胞中 CARD10基因的表达(t=6.83, P<0.01),抑制NF-κB信号通路蛋白的转导。 结论:PEBP1P2在肾细胞癌组织表达明显降低,过表达PEBP1P2可显著抑制肾细胞癌A498细胞的增殖和迁移能力,其分子机制可能是PEBP1P2通过下调 CARD10基因表达抑制NF-κB信号通路转导。Objective:To observe the expression of long non-coding RNA(lncRNA)PEBP1P2 in renal cell carcinoma(RCC)tissues and its effect on the proliferation and migration of RCC cells.Methods:The expression of PEBP1P2 in 51 RCC tissues and RCC cell lines was detected by real-time quantitative polymerase chain reaction(qPCR).The A498 cells with the lowest expression of PEBP1P2 were transfected,and the cells transfected with PEBP1P2 plasmid were used as the PEBP1P2 group,and the cells transfected with the negative control plasmid were used as the NC group.qPCR was used to detect the expression of PEBP1P2 in the two groups of cells.MTT assay and Transwell migration assay were used to detect the proliferation and migration ability of RCC cells.qPCR and Western blotting were used to detect the expression of caspase recruitment domain family member 10(CARD10)gene and NF-κB pathway protein,respectively.Measurement data were expressed as mean±standard deviation(Mean±SD),and LSD-t test was used for comparison between groups.Results:The expression of PEBP1P2 in RCC tissues was lower than that in adjacent tissues(t=4.89,P<0.01).The expression of PEBP1P2 in RCC cells was lower than that in normal renal tubular epithelial cells(P<0.01).The expression of PEBP1P2 in A498 cells of the PEBP1P2 group and NC group was(11.01±1.26)and(1.06±0.19),respectively,and the PEBP1P2 group was significantly higher than that in the NC group(t=7.81,P<0.01).Overexpression of PEBP1P2 significantly inhibited the proliferation of RCC cells(P<0.05)and migration ability(t=3.65,P<0.05).Overexpression of PEBP1P2 significantly suppressed the expression of CARD10 gene in RCC A498 cells(t=6.83,P<0.01)and inhibited the transduction of NF-κB signaling pathway proteins.Conclusions:PEBP1P2 expression was significantly decreased in RCC tissues.Overexpression of PEBP1P2 significantly inhibited the proliferation and migration of RCC A498 cells.Its molecular mechanism is that PEBP1P2 down-regulates CARD10 gene expression and inhibits NF-κB signaling pathway.

关 键 词：长链非编码RNA 肾细胞癌 细胞增殖 细胞迁移 

分 类 号：R737.11[医药卫生—肿瘤]