液液萃取-UPLC快速测定初榨橄榄油中叶绿素铜钠的含量  

Rapid determination of sodium copper chlorophyllin in virgin olive oil by liquid-liquid extraction and UPLC

作　　者：张晓莉 王波 刘阿静 王娟 赵茹玉 金凤 ZHANG Xiaoli;WANG Bo;LIU Ajing;WANG Juan;ZHAO Ruyu;JIN Feng(Gansu Institute of Product Quality Supervision and Inspection,Lanzhou 730010,China;Lanzhou Customs Technology Center,Lanzhou 730010,China;Xiangyu Olive Industry R&D and Analysis Laboratory,Longnan 746000,Gansu,China)

机构地区：[1]甘肃省产品质量监督检验研究院,兰州730010 [2]兰州海关技术中心,兰州730010 [3]祥宇油橄榄产业研发及分析实验室,甘肃陇南746000

出　　处：《中国油脂》2021年第9期149-152,共4页China Oils and Fats

摘　　要：建立了液液萃取-超高效液相色谱(UPLC)快速测定初榨橄榄油中叶绿素铜钠含量的方法。初榨橄榄油样品经80%的乙醇溶液提取后,在UPLC条件为采用SVEA C18色谱柱(50 mm×1.0 mm, 1.7μm),柱温30℃,乙腈-水(体积比95∶5)为流动相,等度洗脱,流速0.3 mL/min,进样量2.0μL,检测波长405 nm下,可实现初榨橄榄油中叶绿素铜钠的有效分离,整个分析过程仅需6 min。通过方法学考察,该方法的检出限及定量限分别为0.20 mg/L和0.50 mg/L,叶绿素铜钠加标回收率均在84.00%~97.90%之间,方法的重复性、精密度和稳定性较好。所建立的方法可用于初榨橄榄油中叶绿素铜钠含量的快速测定。A method for rapid determination of sodium copper chlorophyllin in virgin olive oil by liquid-liquid extraction and ultra-high performance liquid chromatography(UPLC)was established. Virgin olive oil was extracted by 80% ethanol, then injected to ultra-high performance liquid chromatography, and determined under the conditions of SVEA C18 column(50 mm×1.0 mm, 1.7 μm) with isocratic elution at 30℃, acetonitrile-water(volume ratio 95∶5) as mobile phase, flow rate 0.3 mL/min, injection volume 2.0 μL, and detection wavelength 405 nm, the sodium copper chlorophyllin could be effectively separated from virgin olive oil, and the entire analysis process took only 6 min. The results of the methodological examination showed that the detection limit and quantitative limit of the method were 0.20 mg/L and 0.5 mg/L respectively, the recovery rate of sodium copper chlorophyllin was between 84.00% and 97.90%, and the method had better reproducibility, precision and stability, and could be used for the determination of sodium copper chlorophyllin in virgin olive oil.

关 键 词：叶绿素铜钠 液液萃取 超高效液相色谱法 初榨橄榄油 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ646[轻工技术与工程—食品科学与工程]