Longitudinal single-cell RNA-seq of hESCs-derived retinal organoids  

作　　者：Shaojun Wang Sergio Poli Xiaoliang Liang Guang-Hua Peng 

机构地区：[1]Laboratory of Visual Cell Differentiation and Regulation,Basic Medical College,Zhengzhou University,Zhengzhou 450001,China [2]Department of Ophthalmology,First medical center of Chinese PLA General Hospital,Beijing 100853,China [3]Department of Ophthalmology,Fifth medical center of Chinese PLA General Hospital,Beijing 100071,China [4]Division of Pulmonary and Critical Care Medicine,Brigham and Women’s Hospital,Harvard Medical School,Boston 02115,USA [5]Department of Pathophysiology,Basic Medical College,Zhengzhou University,Zhengzhou 450001,China [6]Division of Internal Medicine,Mount Sinai Medical Center,Miami,Florida 33140,USA

出　　处：《Science China(Life Sciences)》2021年第10期1661-1676,共16页中国科学（生命科学英文版）

基　　金：supported by the National Key Research and Development Program(2018YFA0107303);the National Natural Science Foundation of China(82070990);Initiation Fund of Distinguished Professor,Zhengzhou University(32310180);China Postdoctoral Research Fund(2017M613396).

摘　　要：Human retina development involves multiple well-studied signaling pathways that promote the genesis of a wide arrange of different cell types in a complex architectural structure.Human embryonic stem cells(hESCs)-derived retinal organoids could recapitulate the human retinal development.We performed single-cell RNA-seq of retinal organoids from 5 time points(D36,D66,D96,D126,D186)and identified 9 distinct populations of cells.In addition,we analyzed the molecular characteristics of each main population and followed them from genesis to maturity by pseudotime analysis and characterized the cell-cell interactions between different cell types.Interestingly,we identified insulin receptor(INSR)as a specifically expressed receptor involved in the genesis of photoreceptors,and pleiothropin(PTN)-protein tyrosine phosphatase receptor type Z1(PTPRZ1)as a mediator of a previously unknown interaction between Müller and retinal progenitor cells.Taken together,these findings provide a rich transcriptome-based lineage map for studying human retinal development and modeling developmental disorders in retinal organoids.

关 键 词：human embryonic stem cell photoreceptor cell single cell RNA sequencing retinal pigment epithelium retinal organoid 

分 类 号：R774.1[医药卫生—眼科]