Expanding the scope of genome editing with SpG and SpRY variants in rice  

作　　者：Jun Ren Xiangbing Meng Fengyue Hu Qing Liu Yuexuan Cao Huiying Li Changjie Yan Jiayang Li Kejian Wang Hong Yu Chun Wang 

机构地区：[1]State Key Laboratory of Rice Biology,China National Rice Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310006,China [2]State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing),Institute of Genetics and Developmental Biology,The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]Key Laboratory of Plant Functional Genomics,Ministry of Education,Yangzhou University,Yangzhou 225009,China

出　　处：《Science China(Life Sciences)》2021年第10期1784-1787,共4页中国科学（生命科学英文版）

基　　金：supported by the National Transgenic Science and Technology Program(2019ZX08010-003);the Central Publicinterest Scientific Institution Basal Research Fund(Y2020XK17);the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.

摘　　要：Dear Editor,The clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9(CRISPR/Cas9)system,since it was excavated,has been rapidly developed and sparked a revolution in the genome editing field.In principle,CRISPR/Cas9 system relies on the recognition of specific loci on the genome,which is titled the protospacer adjacent motif(PAM).However,the canonical Streptococcus pyogenes Cas9(SpCas9)nuclease only recognizes NGG or NAG PAMs,rendering an inherent obstacle in amplifying the application of CRISPR/Cas9 technology.

关 键 词：Cas9 NUCLEASE RENDERING 

分 类 号：S511[农业科学—作物学] Q943.2[生物学—植物学]