NUCLEASE

作品数：59被引量：330H指数：7
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相关领域：生物学更多>>
相关作者：刘玉彪许馨孙绍光刘静彭国雄更多>>
相关机构：河北医科大学重庆大学河南科技学院广西大学更多>>
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相关基金：国家自然科学基金国家重点基础研究发展计划重庆市自然科学基金中国博士后科学基金更多>>
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Cyanamide-inducible expression of homing nuclease ^(I−)SceI for selectable marker removal and promoter characterisation in Saccharomyces cerevisiae
《Synthetic and Systems Biotechnology》2024年第4期820-827,共8页Liam McDonnell Samuel Evans Zeyu Lu Mitch Suchoronczak Jonah Leighton Eugene Ordeniza Blake Ritchie Nik Valado Niamh Walsh James Antoney Chengqiang Wang Carlos Horacio Luna-Flores Colin Scott Robert Speight Claudia E.Vickers Bingyin Peng 
supported by the Australian Government through the Australian Research Council Centres of Excellence funding scheme(project CE200100029).
In synthetic biology,microbial chassis including yeast Saccharomyces cerevisiae are iteratively engineered with increasing complexity and scale.Wet-lab genetic engineering tools are developed and optimised to facilita...
关键词：Metabolic engineering Genetic circuit Genome engineering Synthetic biology Yeast engineering Promoter engineering 
IsDge10 is a hypercompact TnpB nuclease that confers efficient genome editing in rice
《Plant Communications》2024年第11期6-9,共4页Rui Zhang Xu Tang Yao He Yangcun Li Wei Wang Yawei Wang Danning Wang Xuelian Zheng Yiping Qi Yong Zhang 
Biological Breeding-Major Projects(2023ZD04076)awarded to X.T.and Y.Z.;National Natural Science Foundation of China(award nos.32270433,32101205,and 32072045)to Y.Z.,X.Z.;X.T.It was also supported by the NSF Plant Genome Research Program(IOS-2029889 and IOS-2132693)to Y.Q.
Dear Editor,Cas9 and Cas12a have been widely applied in genome engineering in both plant and human cells(Tang and Zhang,2023).However,the relatively large sizes restrict their delivery into cells via viral vectors.As ...
关键词：SIZES restrict compact 
Origin of the presence/absence variation of the LTIA1/LTIA2 mini-ribonucleaseⅢgenes required for low-temperature growth in rice被引量：1
《The Crop Journal》2024年第5期1459-1470,共12页Jing Yang Yu Peng Limin Mi Aiqing Sun Ping Li Yan Wang Yi Zhang Sheng Teng 
supported by Zhejiang Provincial Natural Science Foundation of China (LD24C130002);Scientific Research Foundation of China Jiliang University。
A rice low temperature-induced albino variant was determined by the recessive ltia1 and ltia2 genes.LTIA1 and LTIA2 encode highly conserved mini-ribonucleasesⅢlocated in chloroplasts and expressed in aerial parts of ...
关键词：RICE Low temperature induced albino Mini-RNaseⅢ Presence/Absence variations Evolution 
Molecular and structural basis of an ATPase-nuclease dualenzyme anti-phage defense complex
《Cell Research》2024年第8期545-555,共11页Qiyin An Yong Wang Zhenhua Tian Jie Han Jinyue Li Fumeng Liao Feiyang Yu Haiyan Zhao Yancheng Wen Heng Zhang Zengqin Deng 
supported by the National Key R&D Program of China(2022YFA1303500);the CAS Pioneer Hundred Talents Program to Z.D.
Coupling distinct enzymatic effectors emerges as an efficient strategy for defense against phage infection in bacterial immune responses,such as the widely studied nuclease and cyclase activities in the type III CRISP...
关键词：ENZYMATIC PLANAR neighboring 
Structure-specific nucleases in genome dynamics and strategies for targeting cancers
《Journal of Molecular Cell Biology》2024年第5期3-18,共16页Haitao Sun Megan Luo Mian Zhou Li Zheng Hongzhi Li R.Steven Esworthy Binghui Shen 
This work was supported by National Institutes of Health(NIH)/National Cancer Institute(NCI)grants(R01CA073764,R01CA085344,and R01CA233664 to B.S.and R50CA211397 to L.Z.);Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(CIFMS,2023-I2M-3-006 to H.S.).
Nucleases are a super family of enzymes that hydrolyze phosphodiester bonds present in genomes.They widely vary in substrates,causing differentiation in cleavage patterns and having a diversified role in maintaining g...
关键词：structure-specific nucleases DNA replication nuclease inhibitors synthetic lethality 
LbCas12a-nuclease-mediated tiling deletion for large-scale targeted editing of non-coding regions in rice
《Plant Communications》2024年第4期141-144,共4页Guigen Ma Fang Yan Bin Ren Zhenwan Lu Hao Xu Fangxi Wu Shaofang Li Daowen Wang Xueping Zhou Huanbin Zhou 
supported by grants from the National Natural Science Foundation of China,China (31871948);the STI 2030–Major Projects (2023ZD04074);the Hainan Yazhou Bay Seed Lab (B21HJ0215);the Central Public-interest Scientific Institution Basal Research Fund (Y2022QC03);the Key Research and Development Program of Shandong Province,China (Agricultural Seed Improvement Project,2022LZGC012);the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences,China。
Dear Editor,This study reports a nuclease-mediated tiling deletion(NTD)method that uses LbCas12a nuclease with a tiling CRISPRderived RNA(crRNA)library to efficiently induce numerous nucleotide deletions in non-coding...
关键词：BREEDING generating NUCLEASE 
An engineered Cas12i nuclease that is an efficient genome editing tool in animals and plants被引量：1
《The Innovation》2024年第2期61-69,共9页Zhiqiang Duan Yafeng Liang Jialei Sun Hongjin Zheng Tong Lin Pengyu Luo Mengge Wang Ruiheng Liu Ying Chen Shuhua Guo Nannan Jia Hongtao Xie Meili Zhou Minghui Xia Kaijun Zhao Shuhui Wang Na Liu Yongling Jia Wei Si Qitong Chen Yechun Hong Ruilin Tian Jian-Kang Zhu 
This work was supported by Shandong BellaGen Biotechnology,and by a grant from the National Natural Science Foundation of China(32188102 to J.-K.Z.).
The type V-I CRISPR-Cas system is becoming increasingly more attractive for genome editing.However,natural nucleases of this system often exhibit low efficiency,limiting their application.Here,we used structure-guided...
关键词：system ATTRACTIVE rational 
C-176 loaded Ce DNase nanoparticles synergistically inhibit the cGAS-STING pathway for ischemic stroke treatment被引量：6
《Bioactive Materials》2023年第11期230-240,共11页Zhixin Zhu Haipeng Lu Lulu Jin Yong Gao Zhefeng Qian Pan Lu Weijun Tong Pik Kwan Lo Zhengwei Mao Haifei Shi 
the National Natural Science Foundation of China(No.22161132027,82272465,and 52273152);Zhejiang Provincial Natural Science Foundation of China(LY20H060008);the Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study(SN-ZJU-SIAS-006);‘Open Competition to Select the Best Candidates’Key Technology Program for Nucleic Acid Drugs of NCTIB(Grant No.NCTIB2022HS02006);Zhejiang High-Level Young Talent Special Support Plan for Dr.Zhengwei Mao.
The neuroinflammatory responses following ischemic stroke cause irreversible nerve cell death.Cell free-double strand DNA(dsDNA)segments from ischemic tissue debris are engulfed by microglia and sensed by their cyclic...
关键词：Ischemic stroke Ce-based nano-nuclease The cGAS-STING signaling pathway Anti-inflammation 
Delivery of an Rhs-family nuclease effector reveals direct penetration of the gram-positive cell envelope by a type VI secretion system in Acidovorax citrulli被引量：1
《mLife》2022年第1期66-78,共13页Tong-Tong Pei Yumin Kan Zeng-Hang Wang Ming-Xuan Tang Hao Li Shuangquan Yan Yang Cui Hao-Yu Zheng Han Luo Xiaoye Liang Tao Dong 
funding from the National Key R&D Program of China(2020YFA0907200);National Natural Science Foundation of China(31770082,32030001).
The type VI secretion system(T6SS)is a double-tubular nanomachine widely found in gram-negative bacteria.Its spear-like Hcp tube is capable of penetrating a neighboring cell for cytosol-to-cytosol protein delivery.How...
关键词：cell envelope cell wall interspecies interaction protein secretion 
The exploration of quantum dot-molecular beacon based MoS_(2) fluorescence probing for myeloma-related Mirnas detection被引量：1
《Bioactive Materials》2022年第11期360-368,共9页Jing Jing Wang Ying Liu Zhou Ding Le Zhang Caiqin Han Changchun Yan Eric Amador Liqin Yuan Ying Wu Chunyuan Song Ying Liu Wei Chen 
The authors acknowledge the generous financial support from the National Natural Science Foundation of China(61971207,61975070,51902143,61775088);Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),Key Research and Development Project of Jiangsu Province(BE2018062,BE2019033);Natural Science Foundation of Jiangsu Province(BK20191467);Post-graduate Research&Practice Innovation Program of Jiangsu Province(KYCX20_2229);International S&T Cooperation Program of Jiangsu Province(BZ2019063,BZ2020045,BZ2020030);Natural Science Foundation of the Jiangsu Higher Education Institutes of China(19KJB430018,20KJA430003);Special Project for Technology Innovation of Xuzhou City(KC19250,KC20201,KC20244);Open Project of State Key Laboratory of Advanced Materials and Electronic Components(FHR-JS-202011017);We also thank Dr.Zhiling Yan for his help and support on the collection of blood samples.WC would like to thank the support from Solgro Inc.,Guangxi Jialouyuan Medical Inc.and UT Arlington distinguished award.
Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS_(2) (QD-MB @MoS_(2)) fluoresc...
关键词：Multiple myeloma miRNA-155 miRNA-150 Duplex-specific nuclease(DSN) Quantum dot MoS_(2) Molecular beacon 
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