LbCas12a-nuclease-mediated tiling deletion for large-scale targeted editing of non-coding regions in rice  

作　　者：Guigen Ma Fang Yan Bin Ren Zhenwan Lu Hao Xu Fangxi Wu Shaofang Li Daowen Wang Xueping Zhou Huanbin Zhou 

机构地区：[1]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China [2]Key Laboratory of Gene Editing Technologies(Hainan),Ministry of Agricultural and Rural Affairs,Sanya 572024,China [3]Scientific Observing and Experimental Station of Crop Pests in Guilin,Ministry of Agriculture and Rural Affairs,Guilin 541399,China [4]Rice Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350019,China [5]State Key Laboratory of Vegetable Biobreeding,National Engineering Research Center for Vegetables,Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,China [6]College of Agronomy,State Key Laboratory of Wheat and Maize Crop Science,and Center for Crop Genome Engineering,Henan Agricultural University,Zhengzhou 450046,China [7]State Key Laboratory of Rice Biology,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China [8]These authors contributed equally to this article

出　　处：《Plant Communications》2024年第4期141-144,共4页植物通讯（英文）

基　　金：supported by grants from the National Natural Science Foundation of China,China (31871948);the STI 2030–Major Projects (2023ZD04074);the Hainan Yazhou Bay Seed Lab (B21HJ0215);the Central Public-interest Scientific Institution Basal Research Fund (Y2022QC03);the Key Research and Development Program of Shandong Province,China (Agricultural Seed Improvement Project,2022LZGC012);the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences,China。

摘　　要：Dear Editor,This study reports a nuclease-mediated tiling deletion(NTD)method that uses LbCas12a nuclease with a tiling CRISPRderived RNA(crRNA)library to efficiently induce numerous nucleotide deletions in non-coding regulatory regions of endogenous rice genes.This method was applied to non-coding regions of the Green Revolution gene SD1,generating 6 mutants with quantitative variations in plant height,which were then used to investigate associations between genotype and phenotype.NTD is thus a promising tool for molecular rice breeding.

关 键 词：BREEDING generating NUCLEASE 

分 类 号：S511[农业科学—作物学]