miR-6886-5p通过调控LASP1表达抑制胃癌细胞的增殖和迁移  

MiR-6886-5p inhibits proliferation and migration of gastric cancer cells by regulating expression of LASP1

作　　者：汤守元[1] 兰国玉 黄耿[2] 朱钟钟 李新明[1] 罗海平[1] 姜进平[1] Tang Shouyuan;Lan Guoyu;Huang Geng;Zhu Zhongzhong;Li Xinming;Luo Haiping;Jiang Jinping(Department of Gastrointestinal and Anorectal Surgery,Huangshi Central Hospital,Edong Healthcare Group(Affiliated Hospital of Hubei Polytechnic University),Huangshi 435000,China;Department of Urology,Huangshi Central Hospital,Edong Healthcare Group(Affiliated Hospital of Hubei Polytechnic University),Huangshi 435000,China)

机构地区：[1]鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)胃肠肛肠外科,湖北435000 [2]鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)泌尿外科,湖北435000

出　　处：《国际医药卫生导报》2021年第19期2973-2976,共4页International Medicine and Health Guidance News

基　　金：湖北省卫生健康科研基金资助项目(WJ2019H158)。

摘　　要：目的观察微小RNA(microRNA,miR)-6886-5p对LIM和SH3蛋白1(LIMandSH3 protein 1,LASP1)表达的调控作用及对胃癌细胞增殖和迁移的影响。方法2019年10月至2021年1月,使用实时定量聚合酶链式反应(qRT-PCR)检测胃癌细胞株和正常胃黏膜上皮细胞中miR-6886-5p表达。以表达最低的细胞为实验对象,分为miR-6886-5p组(转染miR-6886-5p)和对照组(转染miR-NC)。使用淋巴细胞增殖检测(MTS)法和划痕愈合实验分别检测miR-6886-5p对胃癌细胞增殖和迁移能力的影响。使用生物信息学软件microRNA.org及双荧光素酶报告基因实验预测和验证miR-6886-5p的靶基因。使用qRT-PCR和Westernblot检测miR-6886-5p对靶基因表达的调控作用。结果与正常胃黏膜上皮细胞(GES-1)相比,胃癌细胞株miR-6886-5p表达明显下降(P<0.05),表达最低的细胞是HS-746T细胞(P<0.01)。对照组和miR-6886-5p组HS-746T细胞中miR-6886-5p表达分别为(1.17±0.40)和(9.48±1.10),差异有统计学意义(P<0.01)。与对照组相比,miR-6886-5p组细胞的增殖能力明显降低(P<0.05),迁移能力明显降低(P<0.01)。miR-6886-5p可能与LASP1基因的信使RNA(mRNA)存在结合位点。miR-6886-5p可靶向结合LASP1 mRNA(P<0.05)。与对照组相比,miR-6886-5p组LASP1基因表达明显下降(P<0.01)。结论miR-6886-5p在胃癌细胞株中呈低表达,miR-6886-5p能够通过调控LASP1基因表达,降低胃癌HS-746T细胞增殖和迁移能力。Objective To observe the regulation of microRNA(miR)-6886-5p on LIM and SH3 protein 1(LASP1)expressions and its influence on the proliferation and migration of gastric cancer cells.Methods From November,2019 to January,2021,real-time quantitative polymerase chain reaction(qRT-PCR)was used to detect the expressions of miR-6886-5p in the gastric cancer cell lines and normal gastric mucosal epithelial cells.Taking the cells with the lowest expression as the experimental objects,they were divided into a miR-6886-5p group,transfected with miR-6886-5p,and a control group,transfected with miR-NC.The MTS method and scratch healing test were used to detect the effect of miR-6886-5p on the gastric cancer cells'proliferation and migration.The bioinformatics software microRNA.org and the dual luciferase reporter gene experiment were used to predict and verify the target gene of miR-6886-5p.QRT-PCR and Western blot were used to detect the regulation of miR-6886-5p on the target gene expression.Results Compared with that in the normal gastric mucosal epithelial cells(GES-1),the expression of miR-6886-5p in the gastric cancer cell line was significantly decreased(P<0.05),and the expression was the lowest in the HS-746T cells(P<0.01).The expressions of miR-6886-5p in the HS-746 T cells in the control group and the miR-6886-5p group were(1.17±0.40)and(9.48±1.10),respectively(P<0.01).Compared with those of the control group,the cell proliferation and migration of the miR-6886-5p group were significantly reduced(P<0.05 and P<0.01).MiR-6886-5p might have a binding site with the messenger RNA(mRNA)of the LASP1 gene.MiR-6886-5p could targetedly combined with LASP1 mRNA(P<0.05).Compared with that in the control group,the expression of LASP1 gene in the miR-6886-5p group was significantly decreased(P<0.01).Conclusions MiR-6886-5p is low expressed in gastric cancer cell line.MiR-6886-5p can reduce the proliferation and migration of gastric cancer HS-746T cells by regulating the expression of LASP1 gene.

关 键 词：miR-6886-5p LASP1 胃癌 增殖 迁移 

分 类 号：R735.2[医药卫生—肿瘤]