HTAB对污泥发酵效能及微生物种群结构的影响  

Influences of Hexadecyl Trimethyl Ammonium Bromide on the Sludge Fermentation Efficiency and Microbial Community Structure

作　　者：吴丽娟 杨阳 章正勇[1,2] 邹敏 WU Lijuan;YANG Yang;ZHANG Zhengyong;ZOU Min(Jiangsu Key Laboratory of Environmental Engineering,Jiangsu Academy of Environmental Science;Jiangsu Academy of Environmental Sciences Environmental Technology Co.,Ltd.,Jiangsu Environmental Protection Group Co.,Ltd.,Nanjing 210036,China)

机构地区：[1]江苏省环境工程重点实验室,江苏省环境科学研究院 [2]江苏省环科院环境科技有限责任公司,江苏省环保集团有限公司,江苏南京210036

出　　处：《水处理技术》2021年第10期48-52,共5页Technology of Water Treatment

基　　金：江苏省环境工程重点实验室科研开放基金课题(ZX2018005);水体污染控制与治理科技重大专项(2018ZX07208010)。

摘　　要：探究了典型杀菌剂十六烷基三甲基溴化铵(HTAB)对污泥厌氧发酵产短链脂肪酸的影响,采用16S rRNA和16S rRNA(genes)等测序手段分析比较了发酵系统中微生物的种群结构。结果表明,HTAB暴露有效促进了污泥产酸的效能,第6天达到(2 012±101) mg COD/L,相比空白组提高将近4倍,特别是乙酸。进一步发现,HTAB能加速污泥中蛋白质等有机底物的溶解和水解,促进发酵功能微生物的富集,有利于短链脂肪酸的积累。与传统的16S rRNA相比,16S rRNA(genes)测序分析能更加真实、有效地反映发酵系统中功能微生物的重要作用。The effect of typical biocide, hexadecyl trimethyl ammonium bromide(HTAB), on the anaerobic fermentation for short-chain fatty acids(SCFAs) production was studied, and the structure of microbial community in the fermentation system was analyzed and compared by 16 S rRNA and16 S rRNA(genes) sequencing methods. The results showed that, HTAB exposure effectively promoted the SCFAs production efficiency of sludge and the COD reached to(2 012±101) mg COD/L at 6 d, which was nearly 4 times higher than that of the blank group. It was further discovered that,HTAB could accelerate the dissolution and hydrolysis of organic substrates such as proteins in the sludge, promote the enrichment of fermentation function microorganisms, and facilitate the accumulation of SCFAs. Compared with the traditional 16 S rRNA, 16 S rRNA(genes) sequencing analysis can more truly and effectively reflect the important role of functional microorganisms in the fermentation system.

关 键 词：十六烷基三甲基溴化铵(HTAB) 厌氧发酵 剩余污泥 短链脂肪酸 16S rRNA(genes)高通量测序 活性微生物 

分 类 号：X703.1[环境科学与工程—环境工程]