大鼠脑缺血再灌注损伤后脑组织高迁移率族蛋白1表达的变化  

Expression of high mobility group protein box 1 at different time after cerebral ischemia-reperfusion

作　　者：许娜 渠静 王赛楠 江炜 耿晓坤[1] Xu Na;Qu Jing;Wang Sainan;Jiang Wei;Geng Xiaokun(China-America Institute of Neuroscience,Beijing Luhe Hospital,Beijing 101149,China)

机构地区：[1]首都医科大学附属北京潞河医院中美神经研究所,101149

出　　处：《中华脑血管病杂志（电子版）》2021年第5期297-301,共5页Chinese Journal of Cerebrovascular Diseases(Electronic Edition)

摘　　要：目的探讨大鼠脑缺血再灌注损伤后不同时间点脑组织高迁移率族蛋白1(HMGB1)表达的变化。方法40只雄性SD大鼠按随机数字表法分为4组,每组各10只。采用线栓法闭塞大鼠右侧大脑中动脉2 h诱导建立局灶性脑缺血再灌注损伤模型,分别于再灌后6 h、24 h、3 d及7 d后处死各组大鼠。TTC染色检测大鼠脑梗死体积,计算大鼠脑梗死体积比。蛋白质印迹法和ELISA法检测各组大鼠左、右侧脑组织HMGB1蛋白表达水平。RT-PCR检测各组大鼠左、右侧脑组织HMGB1 mRNA水平。结果蛋白质印迹法、ELISA法检测结果均显示,大鼠右侧脑组织HMGB1蛋白水平在脑缺血再灌注后6 h、24 h、3 d及7 d呈逐渐降低的趋势,左侧脑组织HMGB1蛋白水平也呈逐渐降低趋势。缺血再灌注后6 h大鼠右侧脑组织HMGB1蛋白水平高于左侧脑组织,且差异有统计学意义(P<0.001),其他时间点大鼠左右侧脑组织HMGB1蛋白水平差异均无统计学意义(P均>0.05)。RT-PCR检测结果显示,大鼠右侧脑组织HMGB1 mRNA水平在脑缺血再灌注后6 h、24 h、3 d及7 d呈逐渐降低的趋势,左侧脑组织HMGB1 mRNA水平呈先升高、后降低的趋势。缺血再灌注后6 h和24 h大鼠右侧脑组织HMGB1 mRNA水平均较左侧脑组织升高,且差异均有统计学意义(P均<0.001),而其他时间点大鼠左右侧脑组织HMGB1 mRNA水平差异均无统计学意义(P均>0.05)。结论HMGB1表达水平在脑缺血再灌注后6 h至7 d呈降低趋势。Objective To study the expression of high mobility group protein box 1(HMGB1)at different times in cerebral ischemia-reperfusion model of rats.Methods Forty male SD rats were divided into 6 h group,24 h group,3 d group and 7 d group by random number table method.The rat model of focal cerebral ischemia-reperfusion injury was established by occlusion of the middle cerebral artery with thread occlusion for 2 h.The volume of cerebral infarction was measured by TTC staining.The mRNA level of HMGB1 in rat brain tissue was measured by semi-quantitative RT-PCR,and the protein expression of HMGB1 was detected by ELISA and Western blotting at different times after reperfusion in rat middle cerebral artery acclusion(MCAO)model.Results The mRNA level and protein level of HMGB1 decreased at 6 h-7 d after ischemia-reperfusion.The mRNA level of HMGB1 in the right brain tissue was higher than that in the left brain tissue at 6 h and 24 h after cerebral ischemia reperfusion.The protein level of HMGB1 in the right brain tissue was higher than that in the left brain tissue 6 h after cerebral ischemia reperfusion.Conclusion The level of HMGB1 decreased at 6 h-7 d after cerebral ischemia-reperfusion.

关 键 词：脑缺血再灌注 高迁移率族蛋白1 大脑 

分 类 号：R743.3[医药卫生—神经病学与精神病学]