Identification of proteins associated with Fusarium crown rot resistance in wheat using label-free quantification analysis  

作　　者：JIN Jing-jing DUAN Shuo-nan Ql Yong-zhi ZHEN Wen-chao MA Jun 

机构地区：[1]College of Plant Protection,Hebei Agricultural University,Baoding 071001,P.R.China [2]State Key Laboratory of North China Crop Improvement and Regulation,Baoding 071001,P.R.China [3]College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,P.R.China [4]College of Agronomy,Hebei Agricultural University,Baoding 071001,P.R.China

出　　处：《Journal of Integrative Agriculture》2021年第12期3209-3221,共13页农业科学学报（英文版）

基　　金：the National Key Research and Development Program of China(2017YFD0300906 and 2018YFD0300501);the National Natural Science Foundation of China(31872865);the State Key Laboratory of North China Crop Improvement and Regulation.

摘　　要：Fusarium crown rot(FCR),typically caused by Fusarium pseudograminearum,is a severe soil-borne disease that,in recent years,has become an emerging threat to Chinese wheat crops.For the first time in this study,we investigated and compared the proteomic characteristics of two Chinese wheat varieties(04 Zhong 36 and Xinmai 26)at 24,48,and 72 h post-inoculation using label-free quantitative proteomic analysis.A total of 9234 proteins were successfully quantified,of which 783 were differentially expressed after inoculation.These proteins were mainly involved in metabolic,single-organism,and cellular processes.Thirty-three proteins associated with defense,cell wall formation,photosynthesis,etc.,showed consistently different expression between the two genotypes at multiple time points.In particular,chitinase,which degrades chitin in the fungal cell wall and limits fungal growth,was exclusively and consistently upregulated in 04 Zhong 36 across the three time points.Other proteins such as flavonoid O-methyltransferase,glycosyltransferase,and peroxidase were only upregulated in 04 Zhong 36,and proteins,including the berberine bridge enzyme and rubisco large subunit-binding protein,were specifically downregulated in Xinmai 26.The expression of transcripts encoding eight selected proteins through qRT-PCR analysis supported the proteomic profiles.Overall,the results of this study allow us to understand FCR resistance in wheat at the protein level.Some proteins and their corresponding genes may be useful resources for the genetic improvement of FCR resistance in wheat.

关 键 词：WHEAT Fusarium crown rot PROTEOMIC differential expression multiple time points 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]