CdTe量子点—钌配合物—DNA适配体探针检测血小板衍生生长因子  

Detection of platelet-derived growth factor based on Ru complex and CdTe quantum dots as an aptamer probe

作　　者：刘宁益 邓元青 王姗 刘婷 刘学文 Liu Ningyi;Wang Shan;Deng Yuanqing;Liu Ting;Liu Xuewen(College of Chemistry and Material Engineering,Hunan University of Arts and Science,Changde 415000,China)

机构地区：[1]湖南文理学院化学与材料工程学院,湖南常德415000

出　　处：《湖南文理学院学报（自然科学版）》2021年第4期27-30,36,共5页Journal of Hunan University of Arts and Science(Science and Technology)

基　　金：湖南省教育厅重点项目(16A145);湖南省大学生研究性学习和创新性实验计划项目(湘教通[2019]219号)。

摘　　要：血小板衍生生长因子(PDGF-BB)是一种可用于肿瘤早期诊疗的肿瘤标志物,有必要发展新的方法用于PDGF的高灵敏、高选择性检测。本文采用基于巯基丙酸(MPA)修饰的CdTe量子点和钌配合物的适配体探针,建立了一种荧光检测PDGF-BB的方法。在优化条件下,利用适配体与PDGF-BB的特异性结合,该探针可实现对PDGF-BB的荧光检测。探针荧光强度与PDGF-BB浓度的线性范围为6~20 nmol/L,检测限为1.26nmol/L。该方法为量子点—钌配合物—适配体复合体系用于生物活性分子的定量检测提供了新途径。Platelet derived growth factor-BB(PDGF-BB) is a tumor marker for early diagnosis of tumor. Therefore,it is necessary to develop a new method for highly sensitive and selective detection of PDGF-BB. In this work, a new method for fluorescence detection of PDGF-BB has been developed using an aptamer probe based on MPA co-capped CdTe quantum dots(QDs) and ruthenium complex. Under optimal conditions, this probe can detect PDGF-BB using fluorescence based on the specific interaction between apatmer and PDGF-BB. The fluorescence intensity of the sensor displayed a good linear relationship in the concentration range of 6 to 20 nmol/L for PDGF-BB. The detection limit is 1.26 nmol/L. The proposed approach provides a useful method by using QDs-Ru(Ⅱ)-aptamer system for the quantitative detection of various biological molecules.

关 键 词：血小板衍生生长因子 量子点 钌配合物 适配体 荧光探针 

分 类 号：O626[理学—有机化学]