马铃薯StDRO1基因的多态性鉴定及其与根系性状的关联分析  

Identification of StDRO1 gene polymorphism and association analysis with root traits in potato

作　　者：许德蓉 孙超 毕真真[1,2] 秦天元 王一好 李成举 范又方 刘寅笃 张俊莲 白江平[1,2] XU De-Rong;SUN Chao;BI Zhen-Zhen;QIN Tian-Yuan;WANG Yi-Hao;LI Cheng-Ju;FAN You-Fang;LIU Yin-Du;ZHANG Jun-Lian;BAI Jiang-Ping(Gansu Provincial Key Laboratory of Aridland Crop Science/Gansu Key Laboratory of Crop Improvement&Germplasm Enhancement,Lanzhou 730070,Gansu,China;College of Agronomy,Gansu Agricultural University,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070 [2]甘肃农业大学农学院,甘肃兰州730070

出　　处：《作物学报》2022年第1期76-85,共10页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(32060502,31660432);甘肃省创新能力提升项目(2019B-073);国家现代农业产业技术体系建设专项(马铃薯)(CARS-09-P14);甘肃省马铃薯产业体系建设专项(GARS-03-P1);甘肃省科技计划项目(19ZD2WA002-02,18JR3RA174);甘肃农业大学人才专项(2017RCZX-44)资助。

摘　　要：为鉴定与马铃薯根系性状相关联的StDRO1基因单核苷酸多态性(single nucleotide polymorphism,SNP)位点,本研究对110份同源四倍体马铃薯材料StDRO1基因的编码区进行了克隆和测序,筛选其SNP,并对StDRO1的SNP位点与马铃薯总根表面积、总根体积和平均根系直径等主要根系性状参数进行了关联分析。结果显示,StDRO1第2外显子上检测到1个SNP位点,命名为G64C;第3外显子上检测到10个SNP位点,分别命名为G152A、A214G、A297G、C314T、A337T、T353C、T560A、C577A、C620A和C625A;在第4外显子上检测到1个SNP位点,命名为T793A。关联分析结果表明,StDRO1基因G152A位点在总根体积表现为GA类基因型>GG类基因型(P<0.05);C314T位点在平均根系直径表现为CC类基因型>CT类基因型(P<0.05);A337T位点在总根表面积、鲜重和干重均表现为AT类基因型>AA类基因型(P<0.05),而在平均根系直径则表现为AA类基因型>AT类基因型(P<0.05);T353C位点在总根表面积、总根体积和鲜重均表现为TC类基因型>TT类基因型(P<0.05);C620A位点在总根体积表现为CA类基因型>CC类基因型(P<0.05);T793A位点在总根表面积、总根体积和鲜重均表现为AT类基因型>TT类基因型(P<0.05)。综上,StDRO1基因的上述6个SNP对马铃薯根系性状有显著影响,其中A337T、T353C和T793A位点尤为重要。研究结果为后续马铃薯根系构型研究和遗传改良提供了理论参考,但能否作为马铃薯根系性状遗传标记还需扩大群体样本进一步验证。In order to identify the single nucleotide polymorphism(SNP)of StDRO1 gene associated with potato root traits,StDRO1 gene coding region of the 110 tetraploid potato genotypes was cloned and sequenced.The association analysis between the StDRO1 SNPs and the major root traits of potato such as surface area,volume,and average diameter were carried out.The results showed that a SNP(G64C)was detected in the second exon of the StDRO1,10 SNPs(G152A,A214G,A297G,C314T,A337T,T353C,T560A,C577A,C620A,and C625A)were identified in the third exon,and a SNP(T793A)was detected in the fourth exon.The association analysis indicated that in the total root volume,G152A locus of StDRO1 gene showed that GA geno-type was superior to GG genotype(P<0.05).In the average root diameter,C314 locus showed that CC genotype were superior to CT genotype(P<0.05).In the root surface area,fresh weight,and dry weight,A337T locus showed that AT genotype were better than AA genotype(P<0.05),while the locus in the average root diameter showed that AT genotype was significantly lower than AA genotype(P<0.05).The root surface area,root volume,and fresh weight were showed as TC genotype>TT genotype(P<0.05)in T353C site;the root volume was showed as CA genotype>CC genotype(P<0.05)in C620A site;the root surface area,root volume,and fresh weight were showed as AT genotype>TT genotype(P<0.05)in T793A site.In summary,the above six SNPs of StDRO1 genes had a significant impact on potato root traits,and the A337T,T353C,and T793A sites were particularly important.These results provide a basic theoretical reference for subsequent potato root architecture research and genetic im-provement,but whether it could be used as a genetic marker for potato root traits need to be further verified by expanding the population sample.

关 键 词：马铃薯 StDRO1基因 根系性状 关联分析 SNP 

分 类 号：S532[农业科学—作物学]