蛹虫草菌渣中虫草素、多糖、多肽综合提取分离的工艺研究  

Study on Comprehensive Extraction and Separation of Cordycepin, Polysaccharide and Peptide from Cordyceps Militaris Residue

作　　者：雷燕妮[1] 张小斌[1] 陈书存 刘长霞 李多伟[4] LEI Yanni;ZHANG Xiaobin;CHEN Shucun;LIU Changxia;LI Duowei(Shaanxi Qinling Industrial Technology Research Institute of Special Biological Resources,Shangluo,Shaanxi 726000,China;Shangluo Traditional Chinese Medicine Hospital,Shangluo,Shaanxi,726000,China;Xi'an Yumu Biotechnology Research Institute Co.,Ltd.Xi'an,Shaanxi 710068,China;College of Life Sciences,Northwest University,Xi’an,Shaanxi 710069,China)

机构地区：[1]商洛学院陕西秦岭特色生物资源产业技术研究院,陕西商洛726000 [2]商洛市中医医院,陕西商洛726000 [3]西安宇沐生物技术研究院有限公司,陕西西安710001 [4]西北大学生命科学学院,陕西西安710069

出　　处：《陕西农业科学》2021年第9期32-37,共6页Shaanxi Journal of Agricultural Sciences

基　　金：陕西省科技计划项目(2020SF-327);陕西省教育厅服务地方专项计划项目(19JC014);中央引导地方科技发展专项(2019ZY-FP-02)。

摘　　要：[目的]研究从蛹虫草菌渣中综合提取分离虫草素、多糖、多肽的最佳工艺。[方法]以虫草素、多糖、多肽含量为指标,采用正交试验考察提取温度、液料比、提取时间以及提取次数4因素对虫草素、多糖、多肽综合提取得率的影响,并经过膜过滤技术、酶解技术将三者有效地分离。[结果]虫草素、多糖、多肽综合提取分离最佳工艺条件:在95℃下,用16倍量的水热回流提取2.0h,2次,滤液在经过膜过滤技术、酶解技术将三者有效分离。[结论]优选的实验工艺具有提取效率高、省时、省溶剂等优点。To study the best technology for comprehensive extraction and separation of cordycepin, polysaccharides and peptides from cordyceps militaris residue. Taking the contents of cordycepin, polysaccharides and peptides as indexes, an orthogonal experiment was conducted to investigate the effects of extraction temperature, ratio of liquid to material, extraction time and extraction time on the comprehensive extraction rate of cordycepin, polysaccharides and peptides, and membrane filtration technology and enzymatic hydrolysis technology were used to separate effectively cordycepin, polysaccharides and peptides.The optimum conditions for comprehensive extraction and separation of cordycepin, polysaccharides and peptides are as follows: cordycepin, polysaccharides and peptides were processed at 95°C two times by heat reflux extraction with 16 times amount of water for 2.0 hours, and then the filtrate was effectively separated by membrane filtration technology and enzymatic hydrolysis technology. The optimized experimental process has the advantages of high extraction rate, time-saving, solvent-saving and so on.

关 键 词：蛹虫草菌渣 虫草素 多糖 多肽 提取工艺 正交试验法 

分 类 号：S567.3[农业科学—中草药栽培]