乳腺癌患者血清可溶性T细胞免疫球蛋白黏蛋白分子-3与其配体的表达及临床意义  

Expression of Serum Soluble T Cell Immunoglobulin Mucin Molecule-3 and Its Ligand in Breast Cancer Patients and Clinical Significance

作　　者：饶亚华 冯先华[1] 晏黎 崔天盆[1] RAO Ya-hua;FENG Xian-hua;YAN Li;CUI Tian-pen(Wuhan Tnulitional Chinese and Western Medicine Hospital,Wuhan 430022,China)

机构地区：[1]武汉市中西医结合医院,湖北武汉430022

出　　处：《肿瘤学杂志》2021年第9期742-746,共5页Journal of Chinese Oncology

基　　金：国家自然科学基金项目(81770038);湖北省卫生健康科研基金资助(WJ2019Z001)。

摘　　要：[目的]评价乳腺癌患者血清可溶性T细胞免疫球蛋白及黏蛋白分子-3(souble T cell immunoglobulin and mucin-domain containing molecule-3,sTim-3)及其配体半乳糖凝集素-9(galactose lectin-9,Galectin-9)、癌胚抗原相关细胞黏附分子1(carcino-embryonic antigen related cellular adhesion molecule-1,CEACAM-1)表达水平,并探讨其临床意义。[方法]收集2019年7月至2020年10月武汉市中西医结合医院甲乳外科住院的乳腺癌患者90例血清,同期选择选择60名年龄相应匹配的健康体检者血清为对照组。酶联免疫吸附法检测乳腺癌患者和对照组血清sTim-3、Galectin-9、CEACAM-1表达水平。ROC曲线分析血清sTim-3、Galectin-9对乳腺癌的诊断学效能。[结果]乳腺癌患者血清s Tim-3[2.59(1.78~4.03)ng/ml]和Galectin-9[2.20(1.55~3.05)ng/ml]表达水平显著性高于对照组sTim-3[1.79(1.19~2.45)ng/ml]和Galectin-9[1.59(1.18~2.15)ng/ml](P<0.05),乳腺癌患者血清CEACAM-1水平与对照组相比无显著性差异(P=0.622)。乳腺癌患者血清s Tim-3表达水平与年龄、肿瘤大小、临床分期、腋窝淋巴结转移显著性相关(P<0.05),与组织学分级、Ki-67表达无显著性相关(P>0.05)。Galectin-9表达水平与临床病理学特征无显著性相关(P>0.05)。乳腺癌患者血清CEACAM-1表达水平随组织学分级的升高而增高(P=0.025),与其他临床病理学特征无显著性相关(P>0.05)。sTim-3和Galectin-9诊断乳腺癌的曲线下面积分别为0.741(95%CI:0.666~0.816),0.692(95%CI:0.599~0.785)。[结论]乳腺癌患者血清sTim-3及其配体Galectin-9表达水平显著性上调,血清sTim-3与多个临床病理学特征相关,对乳腺癌的诊断具有一定价值。CEACAM-1在乳腺癌患者血清中表达未见升高,但与组织学分级存在一定关联,其与乳腺癌的关系有待进一步研究。[Objective]To analyze the expression levels of soluble T cell immunoglobulin and mucin molecule-3(sTim-3)and its ligand galactose lectin-9(Galectin-9)and carcinoembryonic antigen-associated cell adhesion molecule-1(CEACAM-1)in serum of patients with breast cancer,and to explore their clinical significance.[Methods]The 90 patients with breast cancer were collected from the Department of Thyroid-Breast Surgery,Wuhan Traditional Chinese and Western Medicine Hospital from July 2019 to October 2020.At the same time,the 60 healthy subjects with corresponding age matching were selected as the control group.The expression levels of s Tim-3,Galectin-9 and CEACAM-1 in serum of breast cancer patients and control group were detected by enzyme-linked immunosorbent assay(Elisa).The diagnostic efficacy of serum s Tim-3 and Galectin-9 on breast cancer was analyzed by ROC curve.[Results]The expression levels of serum s Tim-3[2.59(1.78~4.03)ng/ml]and Galectin-9[2.20(1.55~3.05)ng/ml]in breast cancer patients were significantly higher than those in normal control groups[1.79(1.19~2.45)ng/ml]and[1.59(1.18~2.15)ng/ml](P<0.05).There was no significant difference in serum CEACAM-1 between breast cancer patients and normal control group.The level of serum s Tim-3 expression in breast cancer patients was significantly correlated with age,tumor size,clinical stage and axillary lymph node metastasis.The difference was statistically significant(P<0.05),but not correlated with histological grade and Ki-67 expression(P>0.05).There was no significant correlation between the expression of Galectin-9 and clinicopathological data.The level of serum CEACAM-1 expression in patients with breast cancer increased with the increase of histological grade,and the difference was statistically significant(P<0.05).There was no significant correlation with other clinicopathological data(P>0.05).The area under the curve and 95%CI of s Tim-3 and Galectin-9 in the diagnosis of breast cancer were 0.741(95%CI:0.666~0.816)and 0.692(95%CI:0.599~0.785),respectively.[

关 键 词：乳腺癌 T细胞免疫球蛋白黏蛋白分子-3 半乳糖凝集素9 癌胚抗原相关细胞黏附分子1 

分 类 号：R737.9[医药卫生—肿瘤]