超临界CO_(2)萃取梵净山野生百合工艺及组分分析  

EXTRACTION TECHNOLOGY OF WILD-LILY FROM FANJING MOUNTAIN BY SUPERCRITICAL CO_(2) AND ITS COMPONENT ANALYSI

作　　者：梁浩[1] 杜晓静[1] 郁建生[1] LIANG Hao;DU Xiaojing;YU Jiansheng(National&Local United Engineering Research Center of Traditional Chinese Veterinary Medicine Separation and Purification Technology,Tongren Polytechnic College,Tongren 554300,China)

机构地区：[1]铜仁职业技术学院民族中兽药分离纯化技术国家地方联合工程研究中心,铜仁554300

出　　处：《内蒙古农业大学学报（自然科学版）》2021年第5期112-116,共5页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：西部地区创新能力建设项目(发改办高技[2016]2195号);国家民委重点实验室项目(民委发[2019]28号);教育部认定协同创新中心项目(教职成函[2019]10号);铜仁市科技计划项目(铜市科研[2016]17-2号);。

摘　　要：为确定超临界CO_(2)萃取野生百合的较佳条件,通过单因素试验分别对萃取压力、萃取温度、萃取时间、CO_(2)流量、粉碎粒度和夹带剂6个方面进行试验,确定野生百合的最佳萃取工艺为萃取压力20 MPa、萃取温度45℃,萃取时间3.0 h,CO_(2)流量20 L/h,粉碎粒度60目,500 ml无水乙醇为夹带剂。在该条件下对梵净山地区3种野生百合和一种种植百合的鳞茎进行提取,通过气相色谱-质谱联用(GC-MS)法鉴定提取物成分,共鉴定出34种成分,并用峰面积归一化法测定了提取物中各成分的相对含量。In order to determine the optimal conditions for the supercritical CO_(2)extraction of wild-lily,the extraction process,extraction temperature,extraction time,CO_(2) flow rate,pulverization size,and entrainer were tested through single factor experiments.The results showed that the best extraction conditions of the wild-lily were the extraction pressure of 20 MPa,the extraction temperature of 45℃,the extraction time of 3.0 h,the CO_(2) flow rate of 20 L/h,the pulverization particle size of 60 mesh,and with the entrainer of 500 ml anhydrous ethanol.Three kinds of wild-lily and a planting-lily in Fanjing Mountain were extracted by the supercritical-CO_(2) fluid extraction(SFE)method.34 components of lily were identified by the gas chromatography-mass spectrometry(GC/MS)method.The relative contents of the components were investigated through the area normalization method.

关 键 词：百合 超临界萃取 气相色谱-质谱联用 

分 类 号：TS234[轻工技术与工程—粮食、油脂及植物蛋白工程]