HPLC-DAD测定不同采收期壮族药山绿茶中8种酚类成分含量  

Content determination of eight phenols in Ilex hainanensis harvested at different time by HPLC-DAD

作　　者：肖萌 李秀伟 吴霞[1] 赵文文[1] 昝珂[2] 陈筱清 XIAO Meng;LI Xiu-wei;WU Xia;ZHAO Wen-wen;ZAN Ke;CHEN Xiao-qing(School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China;National Institutes for Food and Drug Control,Beijing 102629,China)

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中国食品药品检定研究院,北京102629

出　　处：《中国中药杂志》2021年第19期5038-5043,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81873085,81403183);北京市教委科技计划项目(KM201810025017);北京市教委拔尖人才培育项目(CIT&TCD201904094)。

摘　　要：建立HPLC-DAD同时测定壮族药山绿茶中绿原酸、2-羟甲基-3-羟基-1-丁烯-4-O-β-D-(6″-O-咖啡酰基)-葡萄糖苷、pubescenoside B、芦丁、华中冬青黄酮-7-O-β-D-葡萄糖苷、异绿原酸B、异绿原酸A、异绿原酸C等8种酚类成分含量。以上述8种成分为对照品,采用Waters XBridge C_(18)色谱柱(4.6 mm×250 mm, 5μm),0.5%甲酸水溶液(A)-乙腈(B)为流动相,梯度洗脱(0~8 min, 5%~12%B;8~18 min, 12%~18%B;18~30 min, 18%~25%B;30~40 min, 25%~30%B;40~42 min, 30%~80%B;42~45 min, 80%B),流速0.8 mL·min^(-1),柱温25℃,检测波长282、324、360 nm,进样量5μL。8批不同采收期山绿茶药材中绿原酸、2-羟甲基-3-羟基-1-丁烯-4-O-β-D-(6″-O-咖啡酰基)-葡萄糖苷、pubescenoside B、芦丁、华中冬青黄酮-7-O-β-D-葡萄糖苷、异绿原酸B、异绿原酸A、异绿原酸C分别为0.30~6.29、0.29~3.27、0.15~10.4、0.51~5.85、0.49~9.02、0.51~4.68、1.93~13.4、0.87~5.95 mg·g^(-1)。同时对不同月份采收的样品进行比较,10月份采收的样品中酚类成分含量较高。该研究可用于山绿茶药材中酚类成分的含量测定,为该药材质量标准的完善提供参考。This study intends to develop a high performance liquid chromatography-diode array detection(HPLC-DAD) method for simultaneous determination of chlorogenic acid, 2-hydroxymethyl-3-hydroxyl-1-butene-4-O-β-D-(6″-O-caffeoyl)-glucopyranoside, pubescenoside B, huazhongilexone-7-O-β-D-glucopyranoside, rutin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C in Ilex hainanensis. The HPLC conditions are as follows: Waters XBridge C_(18) column(4.6 mm×250 mm, 5 μm), mobile phase of 0.5% formic acid in water(A)-acetonitrile(B), gradient elution(0-8 min, 5%-12% B;8-18 min, 12%-18% B;18-30 min, 18%-25% B;30-40 min, 25%-30% B;40-42 min, 30%-80% B;42-45 min, 80% B) at the flow rate of 0.8 mL·min^(-1), detection wavelengths of 282, 324, and 360 nm, column temperature of 25 ℃, and injection volume of 5 μL. The content of the 8 phenols in 8 samples was 0.30-6.29, 0.29-3.27, 0.15-10.4, 0.51-5.85, 0.49-9.02, 0.51-4.68, 1.93-13.4, and 0.87-5.95 mg·g^(-1), respectively. Moreover, the content of phenols in the samples collected in October was higher than that of samples harvested in other months. The established method is accurate and sensitive for the determination of phenols in I. hainanensis, which is useful for the quality improvement of this herbal medicine.

关 键 词：山绿茶 HPLC-DAD 酚类 半萜苷 含量测定 采收期 

分 类 号：R29[医药卫生—民族医学]