藏药薰倒牛正丁醇萃取部位降血糖作用研究  

Study on Hypoglycemic Effects of n-butanol Extract from Tibetan Medicine Xundaoniu

作　　者：徐明丽 张艳霞 景明[2,3] 康万荣 李存祥[2,3] XU Mingli;ZHANG Yanxia;JING Ming;KANG Wanrong;LI Cunxiang(Gansu Province Health School,Lanzhou 730000,China;Provincial Key Laboratory of Chemical and Quality Research for Traditional Chinese(Tibetan)Medicine in Colleges and Universities of Gansu Province/Key Laboratory(nurturing station)of Quality and Standard Research for Traditional Chinese Medicines of Gansu Province;Gansu University of Chinese Medicine)

机构地区：[1]甘肃卫生职业学院,甘肃兰州730000 [2]甘肃省高校中(藏)药化学与质量研究省级重点实验室/甘肃省中药质量与标准研究重点实验室培育基地 [3]甘肃中医药大学

出　　处：《西部中医药》2021年第9期65-69,共5页Western Journal of Traditional Chinese Medicine

摘　　要：目的:观察藏药薰倒牛正丁醇萃取部位对2型糖尿病大鼠血糖、血脂及肝脏总超氧化物歧化酶(total superoxide dismutase,T-SOD)、丙二醛(malonaldehyde,MDA)、谷胱甘肽过氧化氢酶(glutathione peroxidase,GSH-Px)的影响。方法:将60只SPF级SD大鼠随机抽取10只为正常组,其余50只用高脂饲料喂养加腹腔注射1%链脲佐菌素(streptozocin,STZ)溶液(30 mg/kg)建立大鼠糖尿病模型,将造模成功大鼠44只随机分为模型组、阳性对照组(二甲双胍0.09 g/mL)及正丁醇萃取部位高(生药量16.6 g/mL)、中(生药量8.3 g/mL)、低(生药量4.15 g/mL)剂量组。灌胃相应药物或生理盐水1 mL/100 g,每日1次,连续30天。心脏取血处死大鼠,检测总胆固醇(cholesterol,CHOL)、甘油三酯(triglyceride,TG)、γ-羟基丁酸(4-hydroxy-butanoic acid,GHB);取心、肝、脾、肾及胸腺称重,计算脏器指数。ELISA法检测肝脏组织匀浆中T-SOD、MDA、GSH-Px含量;取部分肾脏、胰腺,甲醛固定,脱水、包埋、切片、HE染色,做病理学检测。结果:藏药薰倒牛正丁醇萃取部位中、低剂量组能降低糖尿病大鼠血糖及CHOL、TG、GHB、MDA含量,提高GSH-Px含量及超氧化物歧化酶(superoxide dismutase,SOD)活力;中、低剂量组大鼠肾脏、胰腺病理改变与模型组相比有所减轻;各组大鼠各脏器指数比较差异无统计学意义(P>0.05)。结论:藏药薰倒牛正丁醇萃取部位可降低糖尿病大鼠血糖,且对糖尿病大鼠氧化应激有一定减轻作用。Objective:To observe the effects of n-butanol extract from Tibetan medicine Xundaoniu(Bieber steinia heterostemon)on blood glucose,blood lipid,the levels of T-SOD,MDA and GSH-Px in liver of the rats with type two diabetes mellitus(T2DM).Methods:Ten rats were randomly chosen from 60 SPF SD rats and established as the normal group,the remaining 50 rats accepted high fat feed and intraperitoneal injection of 1%STZ solution(30 mg/kg)to establish T2DM rat models,44 successful rat models were randomized into the model group,positive control group(metformin 0.09 g/mL),high(crude drug amount 16.6 g/mL),moderate(crude drug amount 8.3 g/mL)and low(crude drug amount 4.15 g/mL)doses groups of N-butanol extract.They were drenched with the corresponding medicine or physiological saline,1 mL/100 g,once each day,for 30 days consecutively.Rats were killed by taking blood from the heart,to detect CHOL,TG and GHB;the heart,liver,spleen,kidney and thymus were weighed,to calculate organ index.ELISA method was used to measure the contents of T-SOD,MDA and GSH-Px in the homogenate of liver tissue;some kidney and pancreas were taken,fixed with formaldehyde,dehydrated,embedded,sectioned and stained with HE for pathological detection.Results:Moderate and low doses groups of N-butanol extract from Tibetan medicine Xundaoniu could lower the levels of blood glucose,the contents of CHOL,TG,GHB and MDA,raise the contents of GSH-Px and the activity of SOD;pathological changes of the kidney and pancreas in moderate and low doses groups were relieved compared with these of the model group;the difference had no statistical meaning in organ indexes between different groups(P>0.05).Conclusion:N-butanol extract from Tibetan medicine Xundaoniu could lower the levels of blood glucose in T2DM rats,and it could relieve oxidative stress to some extent in DM rats.

关 键 词：2型糖尿病 藏药薰倒牛 正丁醇有效萃取部位 降血糖作用 大鼠 

分 类 号：R917[医药卫生—药物分析学]