牛大力叶中总多糖、总黄酮、总皂苷含量及其抗氧化活性的研究  

Study on the contents of total polysaccharides,total flavonoids,total saponins and the antioxidant activity in the extracts of leaves of Millettia speciosa Champ.

作　　者：莫宏辉 邓国卫 李珊[1] MO Hong-hui;DENG Guo-wei;LI Shan(The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;Hunan Zhonghe Pharmaceutical Co.,Ltd.,Changsha 410205,China)

机构地区：[1]湖南中医药大学第一附属医院,长沙410007 [2]湖南中和制药有限公司,长沙410205

出　　处：《湖北农业科学》2021年第19期88-91,94,共5页Hubei Agricultural Sciences

基　　金：国家中管局“2018级全国中药特色技术传承人才培训项目”(T20184828005);长沙科技局科技项目“湘西土家药宜昌悬钩子抗肝损伤药效物质基础研究”(kq1706060)。

摘　　要：通过热水回流提取法制备牛大力叶提取物,采用显色法结合紫外分光光度法测定该提取物中总多糖、总黄酮及总皂苷含量;同时测定其对DPPH·、ABTS^(+)·、·OH、O_(2)^(-)·自由基的清除能力,以评价体外抗氧化活性。结果表明,牛大力叶的总多糖含量为(1.36±0.05)%、总黄酮含量为(0.61±0.007)%、总皂苷含量为(0.15±0.013)%,对DPPH·、ABTS^(+)·、·OH、O_(2)^(-)·自由基的半数抑制浓度分别为16.11、4.78、10.26、7.51 mg/mL。牛大力叶中不同类型成分的含量不同,以总多糖的含量最高;同时在体外具有较好的抗氧化活性,对ABTS^(+)·的抗氧化作用最强。The extract of leaves from Millettia speciosa Champ.was prepared by hot water reflux extraction,and the contents of total polysaccharide,total flavonoids and total saponins in the extract were determined by colorimetric method combined with UV spectro⁃photometry.At the same time,the scavenging ability of DPPH·,ABTS^(+)·,·OH and O_(2)^(-)·free radical was determined to evaluate the antioxidant activity in vitro.The results showed that,the contents of total polysaccharide,total flavonoids and total saponins of leaves from Millettia speciosa Champ.were(1.36±0.05)%,(0.61±0.007)%,(0.15±0.013)%,respectively.The half inhibitory concentra⁃tions of DPPH·,ABTS^(+)·,·OH and O_(2)^(-)·free radical were 16.11,4.78,10.26 and 7.51 mg/mL.The contents of different types of com⁃ponents of leaves from Millettia speciosa Champ.were different,and the content of total polysaccharide was the highest.At the same time,it had good antioxidant activity in vitro,and had the strongest antioxidant effect on ABTS^(+)·free radical.

关 键 词：牛大力叶 总多糖 总黄酮 总皂苷 抗氧化活性 

分 类 号：R285.5[医药卫生—中药学]