一种适用于微生物学实验教学的改良细菌革兰染色法  

A Modified Bacterial Gram-staining Method Suitable for Microbiologic Experimental Teaching

作　　者：王继璇[1] WANG Jixuan(Zhejiang University,School of Medicine,Hangzhou,Zhejiang 310006)

机构地区：[1]浙江大学医学院,浙江杭州310006

出　　处：《科教导刊》2021年第20期54-56,共3页The Guide Of Science & Education

摘　　要：微生物学实验教学常规细菌革兰染色实验中,常因细菌培养物被反复使用而导致染色结果出现错误。为了提高革兰染色实验教学效果,我们建立了基于10%福尔马林固定菌液的改良革兰染色法。该法染色结果显示,4℃或室温放置5或30 d的福尔马林固定金黄色葡萄球菌、枯草芽孢杆菌、大肠埃希菌菌液不仅具有100%染色正确率,同时还有菌体染色均匀、轮廓清晰、形态典型等优点,不仅提高了革兰染色实验教学效果,同时也明显减少了实验准备工作量及细菌培养基使用量,较好的改进了革兰染色实验教学中存在的问题。In the routine bacterial Gram-staining assay of microbiologic experimental teaching,the wrong staining results frequently occur due to the repeated use of bacterial cultures.In order to improve the teaching effect of bacterial Gramstaining assay,we established a modified Gram-staining method using 10%formalin-fixing bacterial suspensions.The staining results of the formalin-fixed suspensions standing at 4℃or room temperature for 5 or 30 d of Staphylococcus aureus,Bacillus subtilis and Escherichia coli using this modified method not only showed 100%correct rate,but also had the advantages such as uniform staining,edge sharpness and typical shape of bacterial bodies,which improved the teaching effect of Gram-staining assay as well as decrease the work load of experimental preparation and usage of bacterial medium.

关 键 词：微生物实验教学 改良革兰染色法 福尔马林 细菌固定 

分 类 号：Q246[生物学—细胞生物学]