miR-152通过调控CAND1基因促进雪旺细胞迁移的实验研究  

miR-152 promotes Schwann cell migration by regulating CAND1

作　　者：韩峰[1] 刘司达 姜培龙 蒋华军[1] 傅重洋[1] 曲巍[1] Han Feng;Liu Sida;Jiang Peilong;Jiang Huajun;Fu Chongyang;Qu Wei(Department of Hand Surgery,the First Affiliated Hospital of Dalian Medical University,Dalian 116011,China)

机构地区：[1]大连医科大学附属第一医院手显微外科,大连116011

出　　处：《中华手外科杂志》2021年第5期369-374,共6页Chinese Journal of Hand Surgery

摘　　要：目的探讨miR-152对雪旺细胞(Schwann cells,SCs)迁移作用的影响。方法构建大鼠坐骨神经损伤模型,分别于损伤后1、4、7、14 d采用qRT-PCR检测miR-152和CAND1基因在损伤神经中的表达情况;Western blot和qRT-PCR检测miR-152和CAND1在SCs中的表达;Transvvell法测定SCs迁移能力;以焚光素酶报告基因和RNA免疫沉淀法(RNA binding protein immunoprecipitation,RIP)证实miR-152与CAND1的相关性。结果损伤神经近端和远端神经组织中miR-152水平显著升高,miR-152的过度表达促进了SCs迁移,而miR-152抑制剂转染的SCs则明显抑制了细胞迁移。结果提示CAND1是miR-152的靶基因,此外miR-152对CAND1的表达具有负调控作用,CAND1表达上调阻断了miR-152介导的促进SCs迁移作用。结论周围神经损伤后,miR-152可以通过抑制CAND1的表达促进SCs迁移作用。Objective To investigate the effect of miR-152 on the migration of Schwann cells(SCs).Methods The model of sciatic nerve injury was established in rats.The expression of miR-152 and cullin-associated and neddylation-dissociated 1(CAND1)in injured nerve was detected by qRT-PCR at 1,4,7 and 14 days after injury;the expression of miR-152 and CAND1 in SCs was detected by Western blot and qRT-PCR;the migration ability of SCs was determined by Transwell assay;the correlation between miR-152 and CAND1 were verified by Luciferase reporter gene and RNA immunoprecipitation(RIP)assay.Results In the current study,firstly,on the 1st,4th,7th and 14th day after nerve injury,the levels of miR-152 in proximal and distal nerve segments increased significantly.Moreover,overexpression of miR-152 led to the promotion of SCs migration,while a remarkably repression of migration was observed in the cell which were transfected with miR-152 inhibitor.The results suggest that CAND1 was the target gene of miR-152.In addition,miR-152 negatively regulates the expression of CAND1.The up regulation of CAND1 expression blocks miR-152 mediated promotion of SCs migration.Conclusion miR-152 can promote SCs migration by inhibiting the expression of CAND1 after peripheral nerve injury.

关 键 词：周围神经 动物实验 微小RNAS 雪旺细胞 CAND1 

分 类 号：R745[医药卫生—神经病学与精神病学]