柚皮苷对早期釉质龋再矿化的影响  

Influence of naringin on remineralization of early enamel caries

作　　者：刘思瑶 陈红星 黄雨亭 潘爽[1] LIU Siyao;CHEN Hongxing;HUANG Yuting;PAN Shuang(Department of Endodontics,School of Stomatology,Harbin Medical University,Department of Endodontics,the First Affiliated Hospital of Harbin Medical University,Harbin 150000,China)

机构地区：[1]哈尔滨医科大学口腔医学院牙体牙髓病科,哈尔滨医科大学附属第一医院牙体牙髓病科,黑龙江哈尔滨150000

出　　处：《口腔医学》2021年第10期883-887,共5页Stomatology

摘　　要：目的研究不同浓度的柚皮苷溶液对早期釉质龋再矿化的作用.方法通过体外脱矿方法建立人前磨牙早期釉质龋模型,利用不同浓度的柚皮苷溶液作为实验药物,氟化钠(NaF)和去离子水(DW)分别为阳性对照和阴性对照,对样本进行再矿化处理.用电子探针观察和分析釉质表面脱矿前、后的图像变化及钙磷比值.结果电子探针结果显示,去离子水组釉质表面呈典型的脱矿表现,为蜂窝状疏松结构;NaF组可形成均匀球形沉积区;不同浓度柚皮苷溶液组可见不规则沉积物覆盖于脱矿后釉质的蜂窝状表面,并且随着浓度的升高,沉积物由疏松逐渐变为致密.实验组钙磷比值低于NaF组(P<0.05).1、10、20和50 mg/mL柚皮苷溶液组的钙磷比与DW组相似,差异无统计学意义;而100 mg/mL柚皮苷溶液组与1 mg/mL柚皮苷溶液组、10 mg/mL柚皮苷溶液组、和DW组相比有统计学意义(P<0.05).结论不同浓度的柚皮苷溶液可促进早期釉质龋的再矿化,其作用效果随着浓度的升高而增强.Objective To study the effect of naringin solution with different concentration on the remineralization of early enamel caries.Methods Models of early enamel caries of human premolars were established by external demineralization method.The samples were re-mineralized with different concentrations of naringin solution,NaF and deionized water(DW)as positive and negative controls,respectively.The changes of image and the ratio of calcium and phosphorus of melt surface before and after demineralization were observed by electron probe and X-ray energy spectrometer.Results The results of electron probe showed that the enamel surface of the deionized water group showed a typical demineralization,which was a honeycomb loose structure.The NaF group could form a u-niform spherical sedimentary area.Irregular sediments covered the honeycomb surface of the demineralization in naringin solution groups,and with the increase of concentration,the sediments gradually changed from porous to dense.The Ca/P in the experimental group was lower than that in the NaF group(P<0.05).The Ca/P in the 1,10,20 and 50 mg/mL naringin solution groups was similar to that in the DW group,and the difference was not statistically significant.Compared with 1,10 mg/mL naringin solution groups and DW group,100 mg/mL naringin solution group had statistical significance(P<0.05).Conclusion Different concentrations of naringin solution can promote the remineralization of early enamel caries,and the effect of naringin solution increases with the increase of concentration.

关 键 词：柚皮苷 早期釉质龋 脱矿 再矿化 

分 类 号：R781.1[医药卫生—口腔医学]