下咽鳞癌蛋白质谱鉴定及预后靶分子筛选  

Identification of proteins in hypopharyngeal squamous cell carcinoma by mass spectrometry and screening of prognostic target molecules

作　　者：陈立晓 英信江[1] 陈歆维[1] 王菲[1] 孙臻峰[1] 董频[1] CHEN Lixiao;YING Xinjiang;CHEN Xinw ei;WANG Fei;SUN Zhenfeng;DONG Pin(Department of Otorhinolaryngology Head and Neck Surgery,Shanghai General Hospital,Shanghai 200080,China)

机构地区：[1]上海市第一人民医院耳鼻咽喉头颈外科,上海200080

出　　处：《山东大学学报（医学版）》2021年第9期140-147,共8页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(82072989);上海市申康医院发展中心-新兴前沿技术联合攻关项目(SHDC12020120)。

摘　　要：目的利用非数据依赖性采集(DIA)蛋白质组学技术筛选下咽鳞癌晚期肿瘤预后相关的特异性靶分子。方法选取2016年1月至2018年9月在上海市第一人民医院确诊的5对晚期下咽鳞癌的癌组织和癌旁组织,对蛋白组织进行裂解研磨、蛋白电泳、组织酶解、肽段标记、分级标记、高效液相色谱系统分离,采用Q-Exactive质谱仪完成质谱分析。定量分析及生物信息学分析、筛选预后相关蛋白分子。结果建立下咽鳞癌蛋白组学数据库:蛋白质7 395个,肽段63 190个,筛选出的差异蛋白上调36个、下调43个,差异表达蛋白质总数79个。完成层次聚类分析基因本体论和京都基因与基因组百科全书信号通路富集分析、蛋白互做分析,筛选出关键蛋白MRPS5,为下咽鳞癌预后的特异性靶分子。结论 MRPS5可能为下咽鳞状细胞癌的预后靶分子。Objective To screen specific target molecules related to the prognosis of advanced hypopharyngeal squamous cell carcinoma using non-data dependent acquisition(DIA) proteomics technology. Methods A total of 5 pairs of advanced hypopharyngeal squamous cell carcinoma and adjacent tissues diagnosed in Shanghai General Hospital during Jan. 2016 and Sep. 2018 were selected. The protein tissues were lysed and ground. Protein electrophoresis, tissue enzymatic hydrolysis, peptide labeling, graded labeling, and high-performance liquid chromatography were performed. Mass spectrometry was performed with Q-Exactive mass spectrometer. Quantitative analysis and bioinformatics analysis were conducted to screen prognosis protein molecules. Quantitative analysis and bioinformatics analysis were used to screen prognosis related protein molecules. Results A proteomics database was established, including 7,395 proteins, 63,190 peptide segments, and 79 differentially expressed proteins(36 up-regulated and 43 down-regulated). After GO and KEGG enrichment, PPI protein network interaction and prognosis analysis, the key protein MPRS5 was selected as the specific target molecule for the prognosis of advanced hypopharyngeal squamous cell carcinoma. Conclusion MRPS5 may become a prognostic target molecule for hypopharyngeal squamous cell carcinoma.

关 键 词：下咽鳞癌 蛋白质组学 非数据依赖性采集 预后因子 

分 类 号：R739.63[医药卫生—肿瘤]