渤海S油田油藏硫酸盐还原古菌分析  

Sulfate-reducing archaea in Bohai S-oilfield

作　　者：张世仑 王大威[1,2] 尹榆钧 靖波 王秀军[1,2] ZHANG Shilun;WANG Dawei;YIN Yujun;JING Bo;WANG Xiujun(State Key Laboratory of Offshore Oil Exploitation,Beijing 100028,China;China National Offshore Oil Corporation(CNOOC)Research Institute Ltd.,Beijing 100028,China;Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education,College of Life Science,Nankai University,Tianjin 300071,China)

机构地区：[1]海洋石油高效开发国家重点实验室,北京100028 [2]中海油研究总院有限责任公司,北京100028 [3]南开大学生命科学学院分子微生物学与技术教育部重点实验室,天津300071

出　　处：《生物加工过程》2021年第5期562-568,共7页Chinese Journal of Bioprocess Engineering

基　　金：国家科技重大专项(2016ZX05025003)。

摘　　要：为了证实油藏环境中古菌的酸蚀作用,借助高通量测序的方法对渤海S油田古菌的群落组成和多样性进行分析,发现了具有硫酸盐还原功能的古菌:热球菌(Thermococcales)、古球菌(Archaeoglobales)和脱硫球菌(Desulfurococcales)。通过构建aprA、dsrA、dsrB等硫酸盐还原功能基因的分子进化树,获得了这些功能基因在古球菌中的丰度,发现古球菌的占比在dsrA、dsrB中呈现优势,意味着古球菌的主要功能是将亚硫酸盐还原为H_(2)S。另一方面,比较了该油田H_(2)S治理井与非治理井的古菌群落组成,发现热球菌在生物竞争抑制(BCX)作用下,其在古菌中的丰度降幅达到23.84%,表明BCX能够抑制古菌的硫酸盐还原功能。本研究明确了引发硫化氢酸蚀的古菌群和相关的功能基因,为有针对性地开发油田次生H_(2)S治理体系提供依据和指导。In order to confirm the acid-erosion of archaea in deep reservoir,via the high throughput sequencing technology,we analyzed the archaea community composition and diversity in Bohai S-oilfield,revealed three sulfate-reducing archaea Thermococcales,Archaeoglobales and Desulfurococcales.By constructing the phylogenetic trees of sulfate-reducing functional genes aprA,dsrA,and dsrB,we obtained the respective abundance of these functional genes in Archaeoglobales.The percentages of Archaeoglobales were dominant in dsrA and dsrB,leading to a key role in reducing sulfites into hydrogen sulfide.Moreover,we compared the archaea community composition between treatment well and non-treatment well against hydrogen sulfide in the same block.Due to the bio-competitive exclusion(BCX),Thermococcus was down-regulated in abundance by 23.84%,showing a definite suppression towards some sulfate-reducing archaea.Our findings uncovers the genus and relevant functional genes of sulfate-reducing archaea,to facilitate the development of management system against the secondary hydrogen sulfide in oil reservoir.

关 键 词：硫酸盐还原菌 古菌 高通量测序 群落组成 分子进化树 生物竞争抑制 

分 类 号：Q939.97[生物学—微生物学]