华蟾素通过wee1蛋白调控卵巢癌细胞SKOV3的增殖与凋亡  

Cinobufotalin regulating the proliferation and apoptosis of ovarian cancer cells SKOV3 through wee1 protein

作　　者：郭镜 高月[2] GUO Jing;GAO Yue(Department of Rehabilitation Medicine,The Third Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001;Department of Clinical Laboratory,Chinese Traditional Medicine Hospital of Beibei District,Chongqing 400700,China)

机构地区：[1]锦州医科大学附属第三医院康复科,辽宁锦州121001 [2]重庆市北碚区中医院检验科,重庆400700

出　　处：《西安交通大学学报（医学版）》2021年第6期857-861,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：重庆市自然科学基金资助项目(No.cstc2018jcyjAX0100);辽宁省科技厅项目(No.201602326)。

摘　　要：目的观察华蟾素(cinobufotalin, CINO)是否通过抑制wee1蛋白激酶的表达而影响卵巢癌细胞SKOV3增殖与凋亡。方法将pcDNA-wee1转染卵巢癌细胞,构建wee1过表达SKOV3细胞并用Real-time PCR和Western blotting鉴定过表达情况,按10μg/mL CINO干预处理和分组。CCK8法检测细胞增殖情况;流式细胞仪检测各组作用48 h的细胞凋亡情况;Western blotting检测各组作用48 h时Caspase-3、Bcl-2、Bax的表达情况。结果 Real-time PCR和Western blotting检测结果显示,pcDNA-wee1组wee1的表达明显高于空白对照组,而与pcDNA-wee1组比较,CINO+pcDNA-wee1组wee1的表达明显降低。与pcDNA-wee1组相比,CINO+pcDNA-wee1组卵巢癌细胞的增殖明显抑制(P<0.05),细胞凋亡率增加(P<0.05)。Western blotting结果显示,与pcDNA-wee1组比较,CINO+pcDNA-wee1组Bcl-2蛋白表达降低,而Bax和Caspase-3蛋白表达升高。结论 CINO可能通过降低wee1表达而抑制卵巢癌细胞增殖,促进细胞凋亡。Objective To observe whether cinobufotalin(CINO)inhibits the proliferation and improves apoptosis of ovarian cancer SKOV3 cells by inhibiting the expression of wee1. Methods PcDNA-wee1 was transfected into SKOV3 cells. SKOV3 cells that overexpressed with wee1 were constructed and identified by Real-time PCR and Western blotting.After that, 10 μg/mL CINO was used for intervention treatment and grouping. CCK8 assay was used to detect cell proliferation. Cell apoptosis was detected by flow cytometry after 48 h of treatment. Western blotting detected the expressions of Caspase-3, Bax and Bcl-2 in each group. Results Real-time PCR and Western blotting showed that the expression of wee1 was significantly increased in the pcDNA-wee1 group, while the expression of wee1 decreased in the CINO + pcDNA-wee1 group compared with the pcDNA-wee1 group. Compared with the pcDNA-wee1 group, the proliferation of SKOV3 cells in CINO + pcDNA-wee1 group was inhibited(P<0. 05). Compared with pcDNA-wee1 group, the apoptosis rate of SKOV3 cells in CINO+pcDNA-wee1 group was increased(P<0. 05). Western blotting results showed that Bcl-2 protein expression in CINO+pcDNA-wee1 group was inhibited compared with that in pc-DNAwee1 group,while Bax and Caspase-3 protein expressions were enhanced. Conclusion CINO can inhibit the proliferation and improve the apoptosis of ovarian cancer cells by reducing the expression of wee1.

关 键 词：wee1蛋白激酶 华蟾素 细胞增殖 细胞凋亡 卵巢癌 

分 类 号：R737.31[医药卫生—肿瘤]