二氢杨梅素诱导人卵巢癌HO-8910细胞凋亡及机制研究  

Study on the apoptosis induced by dihydromyricetin in human ovarian cancer HO-8910 cells and its mechanism

作　　者：秦安敏 司应明 付盈盈 刘思丽 QIN An-min;SI Ying-ming;FU Ying-ying;LIU Si-li(Department of Obstetrics and Gynecology,Xiangyang Hospital of Traditional Chinese Medicine,Xiangyang Hubei 441000;Department of Obstetrics and Gynecology,The Affiliated Hospital of Hubei University of Arts and Sciences,Xiangyang Hubei 441021,China)

机构地区：[1]湖北省襄阳市中医医院妇产科,441000 [2]湖北文理学院附属医院妇产科,湖北襄阳441021

出　　处：《蚌埠医学院学报》2021年第10期1340-1345,共6页Journal of Bengbu Medical College

摘　　要：目的:二氢杨梅素体外对人卵巢癌HO-8910细胞生长、凋亡的影响,及其作用机制的探讨。方法:体外培养HO-8910细胞,以不同浓度(10、20、40μmol/L)的二氢杨梅素作用于HO-8910细胞,MTT法及CCK-8法检测对细胞生长的抑制作用;AnnexinV-FITC/PI双染流式细胞仪检测对细胞凋亡的影响;Hoechst 33258荧光染色观察细胞凋亡情况;Western blotting法检测Caspase-3、Bcl-2、Bax、ERK、p-ERK蛋白表达情况。结果:MTT和CCK-8结果均显示二氢杨梅素可浓度依赖性地抑制HO-8910细胞的生长;流式细胞仪结果显示二氢杨梅素可浓度依赖性促进HO-8910细胞凋亡;Hoechst 33258荧光染色结果显示,二氢杨梅素作用后的HO-8910细胞呈现典型凋亡形态学改变;Western blotting结果显示二氢杨梅素可上调HO-8910细胞Caspase-3和Bax水平,下调Bcl-2、ERK、p-ERK水平(P<0.05)。结论:二氢杨梅素具有抗人卵巢癌HO-8910细胞活性的作用,并诱导其凋亡,其作用机制与调控ERK/MAPK信号通路有关。Objective:To investigate the effects of dihydromyricetin on the growth and apoptosis of human ovarian cancer HO-8910 cells,and its mechanism.Methods:The HO-8910 cells were cultured in vitro,and treated with dihydromyricetin at different concentrations(10,20 and 40μmol/L).The proliferation of cells was detected using MTT and CCK-8 method,the apoptosis of cells was detected using the Annexin V-FITC/PI flow cytometry and Hoechst 33258 fluorescence staining,and the expression levels of Caspase-3,Bcl-2,Bax,ERK and p-ERK protein were detected using Western blotting.Results:The results of MTT and CCK-8 was showed that dihydromyricetin could inhibit the growth of HO-8910 cells in a concentration-dependent manner.The results of flow cytometry showed that dihydromyricetin could promote the HO-8910 cell apoptosis in a concentration-dependent manner.The typical morphological changes of apoptosis in HO-8910 cells treated with dihydromyrmectin were found using Hoechst 33258 fluorescence staining.The results of Western blotting showed that dihydromyricetin could up-regulate the levels of Caspase-3 and Bax,and down-regulate the levels of Bcl-2,ERK and p-ERK in HO-8910 cells(P<0.05).Conclusions:Dihydromyricetin can inhibit the activity of human ovarian cancer HO-8910 cells and induce their apoptosis,and the mechanism of which is related to the regulation of ERK/MAPK signaling pathway.

关 键 词：卵巢肿瘤 二氢杨梅素 HO-8910细胞 增殖 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]