胰岛移植对糖尿病大鼠周围神经病变的改善作用  

Effect of islet transplantation on diabetic peripheral neuropathy in diabetic rats

作　　者：陈艳艳[1] 李佳[1] 徐自强[2] 夏念格[1] CHEN Yanyan;LI Jia;XU Ziqiang;XIA Nian’ge(Department of Neurology,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China;Department of Transplantation,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China)

机构地区：[1]温州医科大学附属第一医院神经内科,浙江温州325015 [2]温州医科大学附属第一医院移植科,浙江温州325015

出　　处：《温州医科大学学报》2021年第10期806-810,共5页Journal of Wenzhou Medical University

基　　金：温州市科技计划项目(Y20170346)。

摘　　要：目的:观察胰岛移植对糖尿病周围神经病变(DPN)的改善并探讨其可能机制。方法:SD大鼠采用腹腔注射单剂链脲菌素建立糖尿病模型,糖尿病模型建立12周后进行胰岛移植。分为正常对照组、假手术组、糖尿病组、糖尿病胰岛移植组。于糖尿病模型建立后16周,观察各组大鼠痛敏反应时间,采用HE染色观察坐骨神经的病理变化,实时荧光定量PCR检测坐骨神经肿瘤坏死因子α(TNF-α)的mRNA表达,Western blot观察B淋巴细胞瘤2蛋白(BCL-2)、BCL-2相关X蛋白(BAX)、半胱氨酸天冬氨酸蛋白酶3(caspase3)的表达。结果:与糖尿病组大鼠比较,糖尿病胰岛移植组大鼠坐骨神经纤维变性及断裂均有减少,痛敏反应时间明显缩短,坐骨神经内TNFα的mRNA表达明显下降,且BCL-2表达上调,BAX、caspase3表达下调(P<0.05)。结论:胰岛移植后血糖下调,明显改善DPN,可能是通过下调TNFα的分泌、上调BCL-2,下调BAX、caspase3,抑制凋亡来实现的。Objective:To investigate the protective effect of islet transplantation on diabetic peripheral neuropathy and to explore its possible mechanism.Methods:The diabetic model was established by intraperitoneal injection of streptozotocin in SD rats.Islet transplantation was performed 12 weeks after the diabetic modeling.At week 16 after the diabetic modeling,HE staining,mRNA expression of tumor necrosis factorα(TNF-α),expression of B-cell lymphoma-2(BCL2),BCL-2 associated X(bax)and caspase3 were detected in sciatic nerve of each group.Results:After islet transplantation,the peripheral nerve axons and myelin sheath of peripheral nerve were obviously improved.Besides,the withdrawal latency to pain stimuli significantly increased,and the mRNA expression of TNF-αwas obviously lower.Our study has shown upregulation of BCL-2 downregulation of BAX and caspase3 four weeks after islet transplantation,compared with the diabetic group.Conclusion:Islet transplantation can significantly improve the peripheral neuropathy in diabetic rats,probably through the downregulation of TNF-α,via BCL-2/BAX,caspase3 mediated pathways.

关 键 词：糖尿病周围神经病变 炎症反应 胰岛移植 凋亡 大鼠 

分 类 号：R745.4[医药卫生—神经病学与精神病学]