干扰lncRNA SBF2-AS1通过靶向调控miR-582-5p表达抑制乳腺癌MCF-7细胞的增殖、迁移和侵袭  

Downregulation of lncRNA SBF2-AS1 inhibits the proliferation,migration and invasion of breast cancer cell line MCF-7 by targeting miR-582-5p

作　　者：张平弟 宣佶[2] 吴永丰[1] Zhang Pingdi;Zhang Ji;Wu Yongfeng(Jiangbei Branch of Zhongda Hospital Affiliated to Southeast University,Nanjing 210000,China;Nanjing University of Traditional Chinese Medicine,Nanjing 210000,China)

机构地区：[1]东南大学附属中大医院江北院区,南京210000 [2]南京中医药大学,南京210000

出　　处：《广西医科大学学报》2021年第10期1848-1853,共6页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81704196)。

摘　　要：目的:探讨lncRNA SBF2-AS1对乳腺癌细胞恶性生物学行为的影响及其分子机制。方法:乳腺癌细胞MCF-7、SK-BR-3、MDA-MB-231和正常乳腺细胞MCF10A用RPMI-1640培养基常规培养;取对数生长期乳腺癌细胞MCF-7,分别将miR-582-5p模拟物及阴性对照(NC)、SBF2-AS1干扰表达载体(siRNA)及阴性对照转染至MCF-7细胞,记为miR-582-5p组、miR-NC组、si-SBF2-AS1组、si-NC组;将SBF2-AS1干扰表达载体分别与miR-582-5p抑制剂及阴性对照共转染至MCF-7细胞,记为siSBF2-AS1+miR-582-5p组、si-SBF2-AS1+anti-miR-NC组。实时荧光定量PCR(qPCR)检测正常乳腺细胞MCF10A和乳腺癌细胞系MCF-7、SK-BR-3、MDA-MB-231中SBF2-AS1和miR-582-5p的表达水平;MTT法检测MCF-7细胞活性;Transwell检测MCF-7细胞迁移和侵袭数目;双荧光素酶报告基因实验检测SBF2-AS1和miR-582-5p的靶向关系。结果:与正常乳腺细胞HCC1937相比,乳腺癌细胞MCF-7、SK-BR-3、MDA-MB-231中SBF2-AS1的表达水平显著升高,miR-582-5p的表达水平显著降低(均P<0.05)。干扰SBF2-AS1表达或过表达miR-582-5p后,MCF-7细胞活性显著降低,MCF-7细胞中迁移和侵袭数量显著降低(均P<0.05)。SBF2-AS1可靶向调控miR-582-5p的表达。抑制miR-582-5p表达可部分逆转干扰SBF2-AS1表达对乳腺癌MCF-7细胞增殖、迁移和侵袭的作用。结论:干扰lncRNA SBF2-AS1可通过上调miR-582-5p抑制乳腺癌MCF-7细胞的增殖、迁移和侵袭。Objective:To explore the effect and mechanism of lncRNA SBF2-AS1 on the malignant biological behavior of breast cancer cells.Methods:Breast cancer cell lines MCF-7,SK-BR-3 and MDA-MB-231 and normal breast cells MCF10A were cultured in RPMI-1640 medium.Breast cancer MCF-7 cells were transfected with si-NC,si-SBF2-AS1,miR-NC or miR-582-5p mimic,and co-transfected with si-SBF2-AS1 and miR-582-5p inhibitor or inhibitor NC.The cell viability was detected by MTT assay.The expression levels of SBF2-AS1 and miR-582-5p were determined by qPCR.The number of invasive and mitigated cells was measured by Transwell assay.Dual luciferase reporter gene assay was used to detect the targeting relationship between SBF2-AS1 and miR-582-5p.Results:Compared with normal breast cells HCC1937,the expression of SBF2-AS1 in breast cancer cell lines MCF-7,SK-BR-3 and MDA-MB-231 were significantly increased,while the expression of miR-582-5p was significantly decreased(P<0.05).After interfering with the expression of SBF2-AS1 or overexpression of miR-582-5p,the viability,migration and invasion of MCF-7 cells were significantly reduced(P<0.05).SBF2-AS1 targeted and regulated the expression of miR-582-5p.Inhibition of miR-582-5p expression partially reversed the effect of si-SBF2-AS1 on the proliferation,migration and invasion of breast cancer MCF-7 cells.Conclusion:Downregulation of lncRNA SBF2-AS1 can inhibit the proliferation,migration and invasion of breast cancer MCF-7 cells by up-regulating miR-582-5p.

关 键 词：SBF2-AS1 miR-582-5p 乳腺癌 增殖 迁移 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]