红菊苣花色素纯化工艺研究及组分分析  

Purification and composition of anthocyanins from red chicory

作　　者：孙飞龙[1] 宁景叶 王凤娟[1] 原龙[1] 朱琳[1] SUN Feilong;NING Jingye;WANG Fengjuan;YUAN Long;ZHU Lin(School of Environmental and Chemical Engineering,Xi’an Polytechnic University,Xi’an 710048,China)

机构地区：[1]西安工程大学环境与化学工程学院,陕西西安710048

出　　处：《西安工程大学学报》2021年第5期12-18,共7页Journal of Xi’an Polytechnic University

基　　金：陕西省重点研发计划项目(2020NY-118);西安市科技创新引导项目(201805030YD8CG14(19))。

摘　　要：针对红菊苣粗提取物成分复杂、杂质含量高、利用率低等问题,采用多孔微球法对红菊苣花色素进行纯化分析。以红菊苣为材料,对比8种多孔微球对花色素的吸附及解吸性能,确定纯化红菊苣花色素的最佳工艺条件并利用高效液相色谱分析红菊苣花色素的组成成分。结果表明:AB-8型为最佳纯化多孔微球,在2.5 h达到吸附平衡;当上样液pH值为2.0,质量浓度为341.33 mg/L,体积为260 mL时,在体积分数为60%乙醇中解吸1.5 h,纯化后红菊苣花色素其色价从5.3提升到49.1,是未提纯的9.3倍;红菊苣含有6种花色素,其中矢车菊-3-O-葡萄糖苷含量最多。Given the complex composition of red chicory crude extract,the content of impurities,low utilization rate and other issues,the pigment of red chicory was purified by porous microsphere method.Based on the raw material of red chicory,the adsorption and desorption properties of 8 kinds of porous microspheres to the anthodcyamins were compared to determine the best process conditions for purifying the anthocyanins of red chicory and the composition of the anthocyanins was analyzed by HPLC.The results show that the AB-8 type is the best purified microsphere,reaching adsorption balance at 2.5 h.When the pH value of the sample liquid is 2.0,the mass concentration is 341.33 mg/L and the volume is 260 mL,which is the best purification process condition,the volume fraction of desorption ethanol is 60%and the desorption is 1.5 h,the color value of the purified anthocyanins of red chicory pigment increased from 5.3 to 49.1,which is 9.3 times higher than the unpurified.The composition analysis show that the red chicory contains six kinds of anthocyanins,of which the content of chrysanthemum-3-O-glucoside is the highest.

关 键 词：花色素纯化 红菊苣 多孔微球法 高效液相色谱 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]