长链非编码RNA FAS-AS1对胶质瘤细胞的增殖、迁移、侵袭的影响  

Effects of long-stranded non-coding RNA FAS-AS1 on proliferation,migration and invasion of glioma cells

作　　者：孙立波 卞尔保[1] 程梦 张正伟 黄克兵 陈杰 岳小宇 赵兵[1] Sun Libo;Bian Erbao;Cheng Meng(Dept of Neurosurgery, The Second Affiliated Hospital of Anhui Medical University,Hefei 230601)

机构地区：[1]安徽医科大学第二附属医院神经外科,合肥230601

出　　处：《安徽医科大学学报》2021年第11期1745-1749,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省重点研究与开发计划(编号:1804h08020270)。

摘　　要：目的探讨长链非编码RNA FAS-AS1在胶质瘤细胞中的表达及其对胶质瘤细胞增殖、迁移、侵袭的影响。方法使用qRT-PCR分别检测临床标本、胶质瘤细胞系中FAS-AS1表达丰度。采用FAS-AS1质粒转染人脑胶质瘤细胞系U251、SF126细胞,qRT-PCR检测转染效率,通过MTT形成实验及克隆实验检测FAS-AS1对细胞增殖能力的影响;通过Transwell实验检测FAS-AS1对细胞迁移影响;通过基质胶构建生物膜结合Transwell实验检测FAS-AS1对胶质瘤细胞侵袭的影响。结果胶质瘤组织及人脑胶质瘤细胞系中FAS-AS1的表达下调;FAS-AS1质粒转染成功构建过表达FAS-AS1胶质瘤细胞模型,FAS-AS1过表达抑制胶质瘤细胞增殖、迁移和侵袭。结论FAS-AS1在胶质瘤中表达下调并能抑制胶质瘤细胞的增殖、迁移及侵袭。Objective To investigate the expression of long chain non-coding RNA FAS-AS1 in glioma cells and its effect on the proliferation,migration and invasion of glioma cells.Methods The abundance of FAS-AS1 expression in clinical samples and glioma cell lines was detected by qRT-PCR.Human glioma cell lines U251 and SF126 were transfected with FAS-AS1 plasmid.The transfection efficiency was detected by qRT-PCR,the effect of FAS-AS1 on cell proliferation was detected by MTT formation test and cloning test,the effect of FAS-AS1 on cell migration was detected by Transwell assay,and the effect of FAS-AS1 on glioma cell invasion was detected by matrix gel biofilm combined with Transwell experiment.Results The expression of FAS-AS1 in glioma tissue and human glioma cell line was down-regulated;the glioma cell model with overexpression of FAS-AS1 was successfully constructed by FAS-AS1 plasmid transfection,and the overexpression of FAS-AS1 inhibited the proliferation,migration and invasion of glioma cells.Conclusion FAS-AS1 is down-regulated in gliomas and can inhibit the proliferation,migration and invasion of glioma cells.

关 键 词：LncRNA FAS-AS1 胶质瘤 增殖 迁移 侵袭 

分 类 号：R739.41[医药卫生—肿瘤]