小肝癌中14-3-3σ表达的临床意义及与预后的相关性  

Clinical significance of 14-3-3σexpression in small hepatocellular carcinoma and its correlation with prognosis

作　　者：于艳龙 史赢 李强 刘中华 张冉 陈金明 YU Yan-long;SHI Ying;LI Qiang;LIU Zhong-hua;ZHANG Ran;CHEN Jin-ming(Department of Hepatobiliary Surgery,Chifeng Clinical Medical School of Inner Mongolia Medical University,Chifeng 024000,China)

机构地区：[1]内蒙古医科大学赤峰临床医学院肝胆外科,内蒙古赤峰024000

出　　处：《中国现代普通外科进展》2021年第10期777-781,共5页Chinese Journal of Current Advances in General Surgery

基　　金：内蒙古医科大学科技百万工程联合项目(YKD2017KJBW(LH)074)。

摘　　要：目的:确定14-3-3σ蛋白在小肝癌(CHCC)组织中的表达及其与临床意义。方法:采用石蜡包埋组织,进行免疫组织化学染色,分析14-3-3σ蛋白在60例肿瘤直径≤5cm的HCC患者肿瘤、癌旁及正常组织的表达状态。并收集患者临床病例资料,进行随访。探讨其与肝癌临床病理参数之间及生存预后的关系。结果:14-3-3σ在HCC癌组织表达率为61.70%(37/60),与癌旁76.70%(46/60)及正常组织81.7%(49/60)的阳性率相比明显下降,差异有统计学意义(χ^(2)=6.648;P=0.036)。χ^(2)检验单因素分析14-3-3σ蛋白表达率与微血管侵犯(MVI)(χ^(2)=10.440;P=0.001)、病毒定量(χ^(2)=8.061;P=0.005)、AFP(χ^(2)=5.390;P=0.020)、分化程度(χ^(2)=6.515;P=0.038)密切相关,具有统计学意义。Logistic多因素分析认为分化程度(HR 9.261;95%CI:0.930~2.193;P=0.018)是14-3-3σ蛋白表达下降的独立危险因素。14-3-3σ低表达组生存时间及复发时间短于高表达组,差异有统计学意义(P<0.05)。结论:14-3-3σ蛋白在小HCC原发组织中表达下降,可能导致细胞增殖失控,在HCC的发生发展过程中具有重要作用,14-3-3σ蛋白低表达/缺失可能是小HCC患者预后不良的危险因素。Objective:To determine the expression of 14-3-3σprotein in small hepatocellular carcinoma tissues and its significance in clinical prognosis.Methods:The tissues were embedded in paraffin,and immunohistochemical staining was performed to analyze the expression status of 14-3-3σprotein in 60 cases of HCC patients with tumor diameter≤5 cm in cancer,adjacent cancer and normal tissues.And collect the patient's clinical case data for follow-up.To explore the relationship between it and the clinicopathological parameters of liver cancer and survival prognosis.Results:The expression rate of 14-3-3σin HCC cancer tissues was 61.70%(37/60),which was significantly lower than the positive rate of 76.70%(46/60)in adjacent tissues and 81.7%(49/60)in normal tissues.The difference was statistically significant(χ^(2)=6.648;P=0.036).χ^(2)test univariate analysis of 14-3-3σprotein expression rate and MVI(χ^(2)=10.440;P=0.001),virus quantification(χ^(2)=8.061;P=0.005),AFP(χ^(2)=5.390;P=0.020),degree of differentiation(χ^(2)=6.515;P=0.038)are closely related and statistically significant.Logistic regression analysis of the degree of differentiation(HR 9.261;95%CI 0.930~2.193;P=0.018)was an independent risk factor for the decrease of 14-3-3σprotein expression.The survival time and recurrence time of the 14-3-3σlow expression group were shorter than those of the high expression group.There were significant differences,and the differences were statistically significant.Conclusion:The decreased expression of 14-3-3σprotein in the primary tissues of small HCC may lead to uncontrolled cell proliferation and play an important role in the occurrence and development of HCC.The low expression/absence of 14-3-3σprotein may be the cause of small HCC patients Risk factors for poor prognosis.

关 键 词：原发性肝细胞性肝癌 14-3-3σ蛋白 免疫组化 预后 

分 类 号：R73[医药卫生—肿瘤] R44[医药卫生—临床医学]