脑和脊髓动静脉畸形血管中蛋白质表达谱的差异  

Differential protein profiling in brain and spinal arteriovenous malformations

作　　者：王乃利[1] 孟姝[2] 仇文颖[1] 王霞[2] WANG Nai-li;MENG Shu;QIU Wen-ying;WANG Xia(Department of Human Anatomy,Histology and Embryology,Institute of Basic Medical Sciences CAMS,School of Basic Medicine PUMC,Beijing 100005,China;Department of Immunology,Institute of Basic Medical Sciences CAMS,School of Basic Medicine PUMC, Beijing 100005,China)

机构地区：[1]中国医学科学院基础医学研究所北京协和医学院基础学院人体解剖与组织胚胎学系,北京100005 [2]中国医学科学院基础医学研究所北京协和医学院基础学院免疫学系,北京100005

出　　处：《基础医学与临床》2021年第11期1570-1576,共7页Basic and Clinical Medicine

基　　金：国家自然科学基金(81801180);中国医学科学院医学与健康科技创新工程(2016-I2M-1-004)。

摘　　要：目的探讨脑和脊髓动静脉畸形(AVM)在分子水平的表达谱差异。方法提取人脑(bAVM)、脊髓(sAVM)、对照脑血管、对照脊髓血管全蛋白质,通过TMT标记高通量定量蛋白质组学技术检测各组中蛋白质表达水平。结果在bAVM、sAVM、对照脑血管、对照脊髓血管样本中,采用蛋白质组学方法共检测到3102个高可信度蛋白质,在bAVM和sAVM中分别有852个和205个蛋白质的表达水平与其对照组有显著改变。生物信息学分析显示,304个蛋白质仅在bAVM中显著高表达,这些蛋白质主要是线粒体相关蛋白质,主要富集于电子传递链及钙调节通路;97个蛋白质仅在sAVM中显著高表达,主要参与细胞外基质的组成。结论线粒体功能异常及钙调节失衡可能在bAVM畸形血管的病理生理过程中发挥重要作用,细胞外基质组成及其糖基化异常可能参与sAVM畸形血管的进程。Objective To explore the differences of protein profiling between brain arteriovenous malformation(bAVM)and spinal AVM(sAVM).Methods Proteins were extracted from bAVM,sAVM,control cerebrovascular and control spinal vessel specimens.The protein level in each group was measured by TMT-labeled quantitative proteomics.Results Totally 3102 protein samples from bAVM,sAVM,control cerebrovascular and control spinal vessel were examined by high-throughput quantitative proteomics.Among them the expression level of 852 proteins was dramatically changed in bAVM and 205 proteins in sAVM.Bioinformatic analysis showed that 304 proteins were highly expressed only in bAVM,most of them were mitochondria proteins,mainly enriched in electron transport chain and calcium regulation pathways,97 proteins were specifically highly expressed in sAVM associated with extracellular matrix organization.Conclusions The mitochondrial dysfunction and calcium regulation imbalance may play important roles in the process of bAVM.Disturbed extracellular matrix organization and glycoproteins are potentially involved in sAVM progression.

关 键 词：脑动静脉畸形 脊髓动静脉畸形 蛋白质组学 线粒体 细胞外基质 

分 类 号：R363[医药卫生—病理学]