补肾开窍祛痰方药对缺氧缺血性脑损伤大鼠能量代谢及神经细胞的影响  

Effects of Bushen Kaiqiao Qutan Recipe on Energy Metabolism and Nerve Cells in Rats with Hypoxic-Ischemic Brain Injury

作　　者：赵永刚[1] 陈昊[1] 张洪兰[1] ZHAO Yonggang;CHEN Hao;ZHANG Honglan(The First People’s Hospital of Lianyungang City,Jiangsu Province 222000)

机构地区：[1]江苏省连云港市第一人民医院,222000

出　　处：《医学理论与实践》2021年第21期3681-3683,3709,共4页The Journal of Medical Theory and Practice

摘　　要：目的:观察补肾开窍祛痰方药对缺氧缺血性脑损伤大鼠能量代谢及神经细胞的影响。方法:将60只7日龄SD大鼠随机等分为模型组、正常组、假手术组和三个药物组(补肾方0.5倍、1倍、2倍组),每组10只。观察各组大鼠实验前后神经功能损伤、脑组织能量代谢指标及神经细胞变化情况。结果:各组大鼠的脑组织能量代谢指标(AMP、ADP、ATP及能荷值)、神经细胞(BrdU阳性细胞、DCX阳性细胞)分析:与正常组和假手术组比较,模型组、补肾方0.5倍组、补肾方2倍组均下降(P<0.05),与补肾方0.5倍组、补肾方2倍组比较,补肾方1倍组更高(P<0.05)。结论:补肾开窍祛痰方药可显著改善缺氧缺血性脑损伤大鼠神经功能损伤症状,在临床推广中必须给予剂量关注。Objective:To observe the effect of Bushen Kaiqiao Qutan Recipe(Bushen Recipe)on energy metabolism and nerve cells in rats with hypoxic-ischemic brain injury.Methods:607-day-old SD rats were randomly divided into model group,normal group,sham operation group and three drug groups(Bushen Recipe 0.5 time,1 time,2 times group),10 rats in each group.The changes of nerve function,brain energy metabolism index and nerve cell were observed before and after the experiment.Results:The analysis of brain energy metabolism index(AMP,ADP,ATP and energy charge value),nerve cell(BrdU positive cell,DCX positive cell)in each group:compared with the normal group and the sham operation group,the model group,Bushen Recipe 0.5 time group and Bushen Recipe 2 times group all decreased(P<0.05),and compared with the Bushen Recipe 0.5 time group and Bushen Recipe 2 times group,the Bushen Recipe 1 time group of Bushen recipe was higher(P<0.05).Conclusion:Bushen Kaiqiao Qutan prescription can significantly improve the symptoms of neurological damage in rats with hypoxic-ischemic brain damage,We must pay attention to dosage in clinical promotion.

关 键 词：补肾开窍祛痰方 神经功能损伤 能量代谢 缺氧缺血性脑损伤 神经细胞 

分 类 号：R-33[医药卫生]